Complement is part of the bodies natural
immune defence and comprises of 35
biologically active substances.
The complement system forms a reaction
cascade that is triggered by the presence of
immune complexes (classical pathway), cell
INOCULATION OF CULTURE
By Dr. Romona Ndanyi
STREAKING AN AGAR PLATE
Start at one edge of the plate, moving the loop back
and forth while pulling it back to streak about of the
plate. Sterilize the loop, and touch a clean part of the
Training of Somalia Veterinary Staff in Garissa,
15th Feb 7th March 2010
DR. KASIITI JACQUELINE LICHOTI
Enzyme-linked Immunosorbent Assay
Specific interactions between antigen and
Observe and m
Director of Veterinary Services
Dr. Romona Ndanyi (BVM, MSc)
A properly prepared smear accomplishes two things.
Causes bacteria to adhere to a slide so that they can be stained and
Kills them, rendering pathogenic bacteria safe to handle.
Correct density of bacterial ma
Different bacteria exist in nature & their identification
Family: a group of related genera.
Genus: a group of related species.
Species: a group of related strains.
Type: sets of strain within a species (e.g. biotypes , serotyp
Matua Rose (OD MLT, HD
Micro. Msc Immunology)
The survival of microorganisms depends on their ability
to grow under certain chemical and physical conditions.
An understanding of these conditions enables us to
By Dr. Romona Ndanyi (BVM, MSC)
After culture & staining reactions are performed, it may be
necessary to use other tests (biochemical/serological) to identify
Biochemical tests are carried out using pure culture