Lecture 25 (11/7)
Endoplasmic reticulum (I didnt; catch what he was talking about here)
Little dots are ribosomes. The cell secretes things - that is its primary function - so
needs to make extra proteins to secrete.
Ryan Baugh is a good
Lecture 13 (9/28) Test Review
When it gets dark, the plant need nutrients (which it gets through respiration)
Calvin cycle selected for during asteroids/volcanoes (very dark) nuclear winter
Mitchondria in Plants and Animals
No major diff
Lecture 10 (9/19) Metabolism
Links to the Alberts notes ;
How do living organisms extract raw materials and energy from the environment?
How do living organ
Lecture 3 (8/31) - Week 1 Review
If you come to class with a question:
1. Bring your book
2. Know what page you have a question with
1. Nuclei are 7um, cells are 10um. Really?
Cells vary considerably in size.
Lecture 1 (8/27) - Intro7
Bio 201 - 12H27?
Welcome to Bio201
A Gateway to Biology through the Lens of Molecular Biology
Instructors: Dan Kiehart and Alison Hill
Some joke about data and class numbers
1. Introduction and Diversity
Lecture 8 (9/14) - Review #2
Contributors: serrano.sabrina92, rithi.chandy, weiqiaohuang, separk92, elder.yoshida
Not interested in linking numbers, but Lk = Tw + Wr (pg 50,51)
Untangling Linked DNAf
DNA gets tangled, so long and crammed into such a small
Lecture 6 (9/10) - Proteins
The amino acid 'alphabet' gives a lot of possible variation and function.
RNA World Protein World9o
RNA used to be the main catalyst (maybe) "RNA World" but nowadays its mostly proteins.
AA bonded together with peptide bonds ->
Lecture 5 (9/5) - DNA Structure
ICQ This Friday!
The test will cover lecture and lab material to date
The individual ICQ will be followed by a group quiz
Be sure to remember your group number! If you dont, youll lose ten points off your quiz!
Lecture 16 (10/8)-Genes and Transcription
What is a gene? A sequence of DNA bp that codes for something.
But could also code for non-coding RNA
Central Dogma: DNA RNA protein
*DNA can also replicate
We see that not all RNAs going down this path (non-codin
Lecture 20 (10/22)- Eukaryote
Negative regulation: repressor binds to promoter, stop transcription Negative gene regulation
DNA elements are cis-acting, ligands are trans-acting (confirm?) Yes. Trans-acting factors can
Lecture 18 (10/17) - Regulation of
Transcription in Prokaryotes
*if X was missing, what would the effect be?
- non template strand (coding) always the one written (if only one strand given)
- any gene closer to the consensus will be transcribed more than
Lecture 4 (9/3) - DNA is the Genetic
1. If you need extra time on tests / quizzes, get us documentation TODAY
2. Practice Prob. Answers will be posted Tues / Wed
Goals: Diagram, Define, Outline, Distinguish, Understand,
Learn the experiments, sh
Lecture 14 (10/3) Chromosome Dynamics
in Mitosis and Meiosis
Difference between mitosis and meiosis is very simple
Molecular machines made up of protein complexes do really cool things.
Centromere: Helps attach the chromosome to the mitotic spindle The hi
Lecture 15(10/5) - Review
Tip: Try drawing each phase of mitosis/meiosis to understand better
Diploid cell has two chromosomes, one is meacentric and one is telocentric. Really doesnt
matter just helps us keeps things straight.
Each one should come in two
Lecture 2 (8/29) - Biomolecules & Cells
Review session this Friday. No quiz, but its highly recommended that you attend.
Assignment next week.
A site will be set up soon so you can post questions to be answered by the instructor on Friday.
04 November 2015
II Generalized structure of eukaryote protein-encoding genes
III How is specificity achieved in processing mRNAs?
IV Capping the 5 ends of mRNAs
V Polyadenylation and
26 August 2015
1. What is the thread of discovery that led to
DNA as the genetic material?
2. How was RNA discovered and connected as
Gregor Mendel and
Dominant vs recessive
Lecture 9 (9/17) - Nucleic Acid
Southern, Northern Blotting
This week in lab: Gel Electrophoresis
These two paragraphs (below) are about restriction enymes, their sizes and the recognit
Lecture 7 (9/12) - Nucleic Acid
I. Nucleic Acid Purification- Why Solubility matters
II. Enzymatic Manipulations
III. Gel Electrophoresis
IV. Molecular Hybridization
Gene in a goat came from a spider. Milk the proteins out of the goat o.O
Lecture 12(9/26) Chromosomes,
Chromatin and Epigenetics
Stoma in C3 and C4 can close if not there is not enough water. This is done to prevent more
water from being lost through the air. Because the stoma are closed, air can not enter, and CO2
Lecture 17 (10/10) - RNA Transcription
*know the subunits! (alpha, beta, omega) in bac, archea, and euk
Put this on Data Sheet:
RPB1 (Eukaryotes) equiv to Beta (Bacteria
bridge helix- holds jaws together. Oscillation determines movement of RNA p
Lecture 24 (11/2)-Review
non-cognate and cognate - either or not both
Non-cognate: Amino acids not involved in the anticodon on the tRNA
Cognate: Amino acids involved in the anticodon in tRNA
How does 5 capping promote elongation and ter
Lecture 23 (10/31) - RNA
Exam 2 on Monday, 11/5
Recommended video for Translation: https:/www.youtube.com/watch?
v=u9dhO0iCLww#t=180s (3:00 on) <THIS IS THE BEST
Describes sequence changes introduced through se
Lecture 21 (10/24) - Eukaryotic Gene
Contributors: serrano.sabrina92, dianwen, soccersister10, cliu192, sug3duke, jhillrorie,
Mediators is there to serve as an intermediary between gene regulatory proteins and RNA poly
Protein - protei
Lecture 27 (11/12/12) - Translation
Continued Post-Translational Fate of
proteins and a Brief Insight into What
There is a tRNA that has an initiator in the P site
Small subunit binds mRNA with tRNA in P site to the large subunit - In
Lecture 30 (11/26/12) - DNA Damage and
Hi all - my computer is dying so I wont be able to moderate the doc today. Please write
questions for me if you have them and I will answer them later today.
genetic material: store info, express into, replica
Lecture 28 (11/14/12) - DNA Replication I
What is needed for replication:
1. copy 1x only 1 time
2. origins- cis
3. S phase - the cell cycle when DNA is replicated
4. Both strands are copied at the same time
5. 2 Distinct DNA pol complexes with two polyme
Lecture 32 (12/3/12)
transposable elements - changes in the color of corn (the indian corn with the different
colors on each kernel)
knew about mutability before knew what the genetic material was
kinds of transposable elements:
cut and paste - class 2- p
Lecture 11 (9/24) Photosynthesis and
Genetic System of Mitochondria and
How to study for the midterm: Look at the figures and see if you can replicate them in your mind
NADPH - for use in anabolic processes.
Chloroplasts: Responsible for photosyn
Lecture 33 (12/5/12)
The X chromosome has just about perfect syntony
Because presumably, moving the gene off the sex chromosome and to the normal
chromosomes could easily cause problems if not done right, and thus the high level of syntony.
You can use th
Molecular technology II
Livet, et al., Nature 450, 56-62 (1 November 2007)
Visualizing proteins in cells
Cut to m