BIOS E-40- HA #2
DUE DATE: October 11 2016
PART 1: Undergraduate + Graduate, 20 points total.
A tryptic peptide was analyzed with a triple-quadrupole mass spectrometer in ms/ms
mode. The following figure shows the mass spectrum after fragmentation (Pane A
Synopsis for section 2: 2D-gel Electrophoresis.
Become familiar with the ExPASy Swiss 2D-PAGE homepage:
You might want to bookmark the page for easy access.
What does 2D-PAGE stand for?
Bios E-40: Introduction to proteomics!
Translation and post-translational modifications: Part I!
Overview of sorting of nuclear-encoded proteins in eukaryotic cells!
Mitochondrial import: uptake targeting sequences!
1. Rich in
BIOS E-162B Human Pathophysiology II
Sample Problem Set 1 answers
Grads: Answer all questions
Undergrads: Answer questions 1, 2 and 3
You can answer each of the 3 questions with about - 1 of a page of text. Feel free to use original (i.e.
not scanned) dia
1. For each of the following species, indicate whether it is chiral or achiral. For those molecules that are
chiral, circle all stereocenters, and draw the structure of the enantiomer.
BIOS E-40: Introduction to proteomics!
Post-translational modifications: Part II!
In glycoproteins, the carbohydrate side-chains play several roles:!
1. Recognition markers for cell-cell and cell-molecule interactions!
2. Receptors for vi
Synopsis for section 1
Theme: Manipulation of sequences using the NCBI web site and various links.
How do you access to the NCBI web site?
Part A. Introduction to Blast (in class)
Describe the general feature of the blast program and give a
Synopsis for Section 3: Protein Mass Fingerprinting
Open your web browser and perform a google search using the term MASCOT
Select the link http:/www.matrixscience.com
On the top part of the window, select Mascot
A) In this exercise, you will determine th