Chapter 6 (and pp. 200-02)!
Genes, Genomes & Chromosomes!
Exam Pick-up Today! or in Section!
http:/bssa.ucsd.edu/lscw Events began Monday!
Solutions to Exam Problems! will be discussed in sections and ofce hours this week.! There will be no forma
Lecture 9 Expression of Recombinant Proteins
these slides from Lecture 7 (nshing Ch. 5) Review/help schedule Next slide and WebCT
Midterm Exam in 2 locaJons Center 276
Birds Eye View Heading to BIMM 100 MidTe
BIMM100 week 4 (lecture 8)
Tricks for inactivating genes
Stephen Gilmore Graduate Student in Dr. Pillus Lab [email protected]
Midterm Next Thurs(4/29) Peter 108/Center 216 BIG REVIEW Tues 7-9 Pep Can 109 Help Rooms Pac Hall 3501/2 Mon 2-4 Wed 3-5 Fo
BIMM 100 - continuing Chapter 5
(except 5.4) !
Today: ! PCR! hybridization in! all its glory! making good stuff!
Thursday: inactivating genes! * Steve Gilmore!
We will not cover 5.4 before the Midterm!
Additional office hour this week Wednesday 2-3pm in 2
BIMM100 Lecture 4!
On the way to more DNA!
replication & repair processes!
Waitlist has cleared.!
Check WebCT! for problem sets!
Chapter 4! Finished -! n ! ucleic acids! t !ranscription! t !ranslation! Continuing - ! !replication! !damage and repair! !vir
BIMM100 Lecture 3!
Another !big day of RNA! Making more DNA!
Continuing in MCB, Ch. 4!
LP OH starting next week Tues 10:50-noon Mon. 9:30-10:30 2100A or 2130 Pac Hall
Saving Printing? Slides 6-15 from L2
BIMM100 - Lecture 2!
Key Molecular Processes I! A Big Day of RNA! 30 Mar MCB 111-126! 1 April MCB 126-(139) !
(text on [email protected])!
DNA Supercoiling - how it happens!
FAQs next slide
Saving paper? Slides 7-12; 14-19 carry over from Lecture 1 (modif
BIMM 100 Molecular Biology!
Lorraine Pillus! Professor of Molecular Biology! Spring 2010!
Please note the rst few slides of lecture will be
taken from the Syllabus, so are not printed here
Lecture 1 Summary!
Class Overview and Syllabus! N
Lecture 19 Post-transcriptional control, the end.!
(Not covering TOR pathway or eIF2 kinases p. 353-356)! http:/www-biology.ucsd.edu/genericsurvey/studenttaeval.jsp
Ch. 8 Read Classic Experiment 8.1 online ! Catalysis without proteins - the discovery of
Final words about termination! ! !then! Post-transcriptional Regulation!
Anti-termination in Pol II transcription of HIV!
HIV tat encodes a small protein, binds to RNA TAR sequences! TAR has stem-loop structure, bound by bot
BIMM 100 Lecture 18 Post-transcriptional control, contd."
Ch. 8 Read Classic Experiment 8.1 online " Catalysis without proteins - the discovery of self-splicing RNA"
(Not covering TOR pathway or eIF2 kinases p. 353-356)!
Exams available for pickup 3100E P
Lecture 15 - Transcriptional regulation!
Continuing Chapter 7
Parts & Processes!
To detect protein:DNA binding DNaseI footprinting Gel shift (EMSA) assays To detect functional domains Deletion series and in vivo assay
S1 analysis to measure mRNA! Enhance
Chapter 6 !end - Key terms and questions except 7, 8, 15!
The Big Chapter 7!
OH this week Mon and Wed (2-3pm)
No Tuesday OH
Todays Experiments and Techniques biochemical definition of 3 distinct RNA polymerases defining important sequences for transc
L13 BIMM100 - nishing Ch. 6; begin Ch. 7!
Chromosomes and Genomes! on to! Regulation of Expression! Todays experiments & techniques! dening functional elements of chromosomes! determining that the RNA component of telomerase! !provides template informatio
BIMM100: Lecture 12 continuing Chapter 6
Nature Genetics, May 2009
Chromatin and Chromosomes
Still dont have your midterm? Pick up exams from K. McPherson 3100E Pac Hall, 8-11 M-F New techniques: DNase I digestion as a probe for chromatin structure (Fig 6