CHEM114C Biosynthesis of Macromolecules
Prof. Simpson Joseph
Spring 2016
University of California, San Diego
Midterm 2: May 16, 2016
KEY
Name (Print):
UCSD PID:
TA:
Section:
TOTAL QUESTIONS = 23
Answer ONLY in the space provided next to each question.
Max
Chapter 8: Enzymes Basic
Concepts and Kinetics
Transition State Analogs
1948 Linus Pauling proposed that compounds that resemble the
transition state of an enzymatic reaction should be very effective
inhibitors
Called transition state analogs
Eg. Proline
10/18/13
Exploring Proteins and Proteomes
Atoms used in NMR
Only some nuclei give rise to an NMR signal
1
10/18/13
Two-Dimensional NMR
Two-dimensional NMR can be used to determine the 3D
structure of a protein
We examine how the spins on different
10/9/13
Exploring Proteins and Proteomes
What is the Proteome?
Proteome the full complement of proteins expressed from an
organisms genome
The genome provides a list of protein products that could be
present, however only a subset will be expressed in
Protein Composition and Structure
Central Dogma
DNA
Genotype
RNA function &
structure
Protein sequence
RNA
Protein structure
Protein
Protein Function
Phenotype
Proteins
Proteins are linear polymers built of monomers called amino
acids
Protein
The University of California at San Diego
Chemistry 114A Syllabus Fall 2013
Lectures: Tuesday, Thursday 9:30-10:50AM at YORK Hall 2722
Instructor: Patricia A. Jennings
Office: 3111 Natural Sciences Building
Email: [email protected] (NOTE: When
10/25/13
Chapter 8: Enzymes Basic
Concepts and Kinetics
Active Site
Active site of a protein is the region which binds the substrates
and any required cofactors
Contains residues directly required for making or breaking
chemical bonds these are called t
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Biochemistry has become the foundation for understanding all
biological processes. It has provided explanations for the causes of
many diseases in humans, animals and plants."
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How do you know the size of a
protein?
Zonal centrifugation (sedimentation
coefcient)
SDS-PAGE (electrophoresis)
Mass Spectroscopy
1
2
Sedimentation coefcient
s = m (1 - )/f
3
s = sedimentation coefcient
m= mass
= partial specic volume
= density of
Exploring genes and genomes
(Chap. 5)
DNA manipulation techniques
Nucleic Acids
Technical tools
Recombinant DNA technology
Genomes
Eukaryotic genes
1
Exploring genes and genomes
(Chap. 5)
DNA manipulation techniques
2
What is recombinant DNA
technolog
CHEM114C Biosynthesis of Macromolecules
Prof. Simpson Joseph
Spring 2016
University of California, San Diego
Midterm#1: April 18, 2016
Name (Print):
UCSD PID:
TA:
Section:
TOTAL QUESTIONS = 20
Answer ONLY in the space provided next to each question.
Maxim
Final Exam
-Final Exam on Dec. 10
-will cover chapters 1-13
1. Chapters 1 to 8 40%
-amino acids and nucleotides
-DNA, RNA, proteins structure and replication
-recombinant DNA techniques
-hemoglobin and myoglobin
-enzyme kinetics
Final Exam (cont.)
2. Chap
