Laboratory 3: Isolation and Restriction Mapping of Plasmid DNA
Background Reading in DNA Science, 2nd Ed.:
Chapter 4: 108 119
Laboratory 3: 351 361
Laboratory 6: 423 - 441
Objectives of Laboratory 3:
1. Purify two different plasmid DNAs (pRAS2 and pUC19)
Chapter 9: Chromosomes, the Cell Cycle, and Cell Division TEST FILE QUESTIONS
Multiple Choice 1. HeLa cells, which have been used for more than fifty years in biomedical research, a. have led to a cure for certain cancers. b. can live and multiply outside
C ase 2
Histidine-Proline-rich Glycoprotein as a Plasma pH sensor
1. a. Since heparin is negatively charged, HPRG will bind to heparin more effectively if the HPRG is
positively charged. The pKa of histidine is around 6.0. Below this pH, histidine is prot
At the end of this lab, you should be able to:
1. Describe how restriction enzymes work.
2. Explain how DNA ngerprinting works.
3. Understand how gel electrophoresis works.
A. DNA Fingerprinting
DNA ngerprinting takes advantag
Investigating Gas Chromatography
Gas Chromatography is a technique widely used to separate complex mixtures of substances.
Compounds present in a volatile liquid or gaseous solute are isolated after traveling through a
coated column based on the substance
Activity Myoglobin and Hemoglobin
Myoglobin and hemoglobin have different functions. The structure of each protein is
related to its function.
Oxygen (O2) binds to hemoglobin cooperatively
Modulators can bind to hemoglobin and af
pH MEASUREMENTS BUFFERS AND THEIR PROPERTIES
One of the more important properties of an aqueous solution is its concentration of hydrogen ion. The H+ or H3 O+ ion has great effect on the solubility of many inorganic and organic species, on t
Biology: Ecology Unit Test
1. Populations of K-strategists grow rapidly, while r-strategist populations grow slowly.
2. Natural selection acts on phenotypes, not genotypes.
3. In stabilizing selection, the range of phenotypes becomes wider.
SI 10/19/08 Exam 2 Review
1. Which of the following phylogenetic groups within the animal kingdom encompasses all the others in the list? A. rotifera B. deuterostomes C. bilateria D. arthropoda E. protostomes 2. Octopi are most closely related to which of
Experiment 4 The Gram Stain
Gram stain is used to differentiate types of bacteria depending on their abilities to retain a
Differential staining technique differentiating bacteria types by observing the amount
of stain they abso
B IO 304 Sections 001, 002, 003
H omework #1
1 . An organism has a diploid number of 30.
a) In the dividing somatic cells of this organism, how many chromatids are visible at the end of
prophase of mitosis?
b) How many chromosomes are moving to each po
Lab Report on Diraction and Interference
Michael Goerz, Anton Haase
30. September 2005
Tutor: M. Fushitani
We will do the experiment using the He-Ne-laser instead of the Na-lamp.
The laser that we will use is a 4-level-las
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Apr. 2010, p. 23772386
Copyright 2010, American Society for Microbiology. All Rights Reserved.
Vol. 76, No. 8
Effects of Simulated Mars Conditions on the Survival and Gro
Bellevue College | CHEM& 161
Titration of Aspirin Tablets
In this lab, you will determine the percent purity of two commercially available aspiring tablets using an
acid-base titration. In general, an acid and a base react to produce a salt and water by t
502 L6 Genetic Screens
Molecular Genetics 502 Lecture 6
Forward and Reverse Genetic Screens
An example of a genetic screen and genomic database search in yeast
1. Design the experiment.
a. Determine the assay: Screen or Selection?
i) Screen: Search throug
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Dr. J. L. Lyle
Office Hours: Will be announced in class; open door policy
email: [email protected]
Introduction to Organic Laboratory
THE NORMAL DISTRIBUTIONS
Use the following density curve to answer questions 1 3.
1. For this density curve, the third quartile is
2. For this density curve, what percent of the observations lie below 0.5?
Montgomery College Takoma Park Silver Spring Campus
CH203 Organic Chemistry I
Practice Exam II
Problem I (20 points)
Consider the following SN1 reaction:
(a) Draw an energy diagram for the reaction. L
Two Categorical Variables:
The Chi-Square Test
In this chapter, we describe how to use the TI-83/84 Plus to perform a chi-square test on data
in a two-way table. We shall be testing whether the categories of traits listed as the row
Chapter 10: Genetics: Mendel and Beyond TEST FILE QUESTIONS
Multiple Choice 1. In Klreuters studies, reciprocal crosses a. always gave identical results. b. only involved heterozygous individuals. c. supported the blending hypothesis of inheritance. d. co