LAB PRACTICAL REVIEW SHEET
LAB PRACTICAL II
This tests the ability of an organism to ferment different carbohydrates into
acid and perhaps liberate gas.
Durham tube apparatus-a large tube containing Phenol
1. This anaerobic pathway oxidizes glucose to pyruvic acid.
2._ lower the activation energy necessary for biochemical reactions to occur.
1. The Gram stain differentiates bacteria types on the basis of the structure of the
2.Which of the following scientists performed similar
1. This term refers to the combination of the cell membrane(s), wall and any external structure associated with them.
2. A cell membranes are composed primarily of
1.Which of the following statements concerning transcription of bacterial mRNA is not true?
1. A nurse is teaching parents of preschoolers appropriate safety measures for this age
group. Which of the following might be a focus of the teaching plan?
A) Childproofing the house
B) Smoking cessation
C) Fire safety
D) Gun safety
2. Which of th
Investigations in Microbiology:
A Laboratory Manual
Karl J. Roberts, Ph.D., Natalya Koval, MD., Lloyd McAtee, Ph.D.
and Nancy Yurko, Ph.D.
The following rules and regulations must be strictly followed to ensure safe
1. Which of the following are the purposes for collecting specimens? Select all that apply.
A) Screen for health problems.
B) Cure health problems.
C) Diagnose health problems.
D) Treat health problems.
E) Direct the plan of care.
F) Prevent nosocomial in
1. A nurse is administering insulin to a diabetic patient. Which of the following are three
recommended times to check the label before administration? Select all that apply.
A) When reaching for the container or unit dose package
B) After retrieval from
1. What is the body mass index (BMI) of a patient who is 1.68 meters tall and weighs 70
2. A physician orders nutritional therapy administered via a central vein for a patient who
cannot take foods orally. What is the t
1. A nurse is measuring the intake and output of a patient who is dehydrated. What is the
average adult daily fluid intake in milliliters that the nurse would use as a comparison?
A) 1,500 mL
B) 1,800 mL
C) 2,300 mL
D) 2,600 mL
2. A nurse is caring for a
1. A nurse performs a general survey on a patient who is being admitted to the hospital for
COPD. Which of the following are components of this type of assessment? Select all
A) Vital signs
C) Lab tests
1. Which of the following is the most reliable method for assessing pain?
A) Asking significant others
B) Asking the primary care provider
C) Asking the patient
D) Observing the patient
2. A nurse is caring for a 2-day-old neonate in a NICU. What pain ass
1. A nurse assesses the vital signs of a healthy newborn infant. What respiratory rate could
be expected based on the developmental level of this patient?
A) 15 to 25 breaths/minute
B) 16 to 20 breaths/minute
C) 20 to 40 breaths/minute
D) 30 to 60 breaths
ASEPSIS AND INFECTION CONTROL
1. Nurses and other healthcare workers play a key role in reducing the spread of disease,
minimizing complications and reducing adverse outcomes for their patients. Which of
the following statements accurately describe this p
human blood. The blood is collected from the previously examined
donor into a sterile blood bag (transport packaging) and stored with an
added preservative as whole blood. At 387 present, the most commonly
used blood bags triple and quadruple blood bag sy
closed system Aids Vacuette closed sampling system Protective
gloves Protective pad Sterile gauze swabs or squares Antiseptic
disinfectant solution Esmarch bandage, tourniquet Kidney bowl
Plaster tape Waste bin (container) Labelled plastic or glass tubes
anticipated greater blood loss when the patient donates blood for
themselves (autotransfusion) in advance as a reserve. This autologous
blood donation serves for the use of blood by the donor. The blood is
stored for 35 days at 2-6 C. Red blood cell prepa
sampled from a blood vessel directly into the test tube (glass, plastic),
vials, heparinized micro-tubes, micropipettes or on the screening test
strips. Fig. 14.1-2: Aids used in an open system blood collection Aids
The basic set of aids is complemented w
blood gases according to Astrup 408 Collection of: Capillary blood:
into 2 heparinized capillary tubes, Arterial blood: 1 ml of native blood
into heparinized syringes. Transport: In a horizontal position with
crushed ice Basic parameters: pH, pO2, pCO2, S
What is the storage temperature for blood plasma? What is the storage
temperature for erythrocyte preparations? What is the storage
temperature for platelet preparations? What is the expiration time for
blood plasma when stored correctly? How are the eryt
principle adopted by a nurse to limit the risk to their own health during
the collection of biological material: Puts the cap back on the used
needle that is to be discarded Used needles and other disposable
sampling aids and material are disposed of in t
30 to 120 min. after transfusion and is manifested with chills, fever to
febris, tachycardia, nausea, vomiting. It is the most common
complication. First aid: Stop the transfusion, call the doctor, measure the
physiological functions, cover the patient an
J. a kol. Oetovatelstv pro stedn zdravotnick koly 2. ronk. 2. dl.
1. vydn, Praha: Grada Publishing, a.s., 2009. 236 s. ISBN 978-80-2473106-3 KOZIEROV, B., ERBOV, G., OLIVIEROV, R.
Oetrovatestvo I. a II. dl. Martin: Osveta, 1995. 1474 s. ISBN 80-2170528-0
Donor identification number ABO blood group, RhD Guarantee of
negative examination tests Composition and volume of preservative
solution Transfusion preparation volume Date of collection
Expiration date Storage instructions Distinguished labels for RhD
(washed erythrocytes) plasma is replaced by a solution. 388 Fig. 13.72: Erythrocytes resuspended leukodepleted (ERD) Fig. 13.7-3: Storing
erythrocytes transfusion products in a special cooling box Platelet
preparations used to adjust thrombocytopenia and
complications. Describe the most common complications (posttransfusion reaction) that can occur during or after a transfusion.
Theoretical notes Transfusion is defined as the transfer of human blood
or its components from one person (the donor) into the b
and go to the toilet after 8 hrs. (The patient can get up and go to the
toilet even earlier than after 8 hours if an atraumatic needle was used for
the lumbar puncture). The patient is given at least two litres of fluid
(preferably water or tea; currently
diagnostic analysis of the blood components impairment precedes the
indication of the transfusion. The doctor is responsible for administering
the transfusion product and for monitoring the patient (15 20 min.) at
the start of the transfusion. The nurse c
to the infusion. Make a record of the infusion administration in the
documentation. When replacing the infusion bottle and the infusion set,
the working procedure is identical to the procedure for preparing the first
infusion bottle. Make sure the ends of
and stored away. In the event the transfusion component is sent back the
blood bank due to serious reasons, a new blood sample from the patient
is sent together with the form. Complications The complications include
all adverse reactions - post-transfusio
shelf-life of the fresh frozen plasma depends on the storage temperature.
If the storage temperature is -25 or more (up to -40 C), the expiration
period is 36 months (see Fig. 13.7-5). The plasma must be defrosted
before use in a water bath with a maximum
many drops/min) is set for infusion if the indication is 500ml G 5% for 4
hours? Indicate two basic principles regulated by the infusion pumps.
List the solutions replacing amino acids. Indicate the correct order of
the individual steps when administerin