model organism: sf21 organism
growth curves: time (hours) and cell density (cells/mL)
specific growth rates (meu)
pop doubling time
ddNTPs and dNTPs: how are they similar/different
know overview of this semester: steps from DNA extraction to DNA sequence
Proper use of equipment, pipetting, and aseptic technique
The purpose of the lab is to understand basic laboratory safety rules and practice using the
pipette with large and small volumes. As an example, students performed a dilution series to
Lab 7: Gels of PCR reaction, cloning of PCR product
The following agenda focuses on todays lab:
instructor summary comments
load gels with PCR product
perform cloning ligation reaction with PCR product
Lab 9-10: cDNA cleanup and Sanger Sequencing
Labs 9 and 10 aim to accomplish the following:
potential in-class quiz
completion of Lab 9
copy/paste a sequence into tBLAST of the NCBI database
Sanger sequencing is the
Lab 8: Clone Screening
Todays objective is to complete an in-class quiz, receive instruction from the TA, and
begin screening our clones for successfully ligated DNA and full transformation of E. Coli.
In the past few weeks, we have
Lab 6: DNA Extraction and Quantification, Prepare your primers*, Set up PCR
An objective of this lab is to extract DNA from our sample cells which must be
measured through spectrophotometry. Then a DNA primer should be prepared based
Lab 5: Starting Lab Practicum
The purpose of this lab is to accomplish the following:
Complete a quiz
Learn how to navigate through NCBI database while documenting our work.
Identify our gene of interest.
Design oligonucleotide primers.
The labs objective is to begin incubations for the MTT assay, review last weeks data, passage
cells with Trypan blue cell densities, and finish the MTT assay by recording the readings.
During last weeks lab, Trypan blu
Apoptotic Activity Measurement on UV treated Sf21 Cells
The purpose of this lab is to execute the Caspase 3 Apoptotic Assay for UV treated cells.
There will be a two hour incubation which students will perform cell counts and passage Sf21
The lab is intended to test our pipetting skills, review data from last weeks sterility test and
plating, and understand the model system. In learning about growth measurements, we will split
a culture into a flask to begin