3. The -subunit of the G proteins and the -subunit of ATP synthase are examples of
the same family of proteins, the P-loop NTPase family. The NTP (nucleotide)
binding domain contains a central -sheet surrounded on both sides by -helices.
The P-loop, which
linear phage DNA enters the cell with wrapping of DNA around a 515: I i H i3 oligomer
p3 terminal protein (TP) bound to each 5'end leads to helix stabilisation at the DNA ends
phoephcester-bcnd between WP and the 'pare
The Solid Support - CPG
The support used in the DNA synthesis is controlledpore glass (CPG) and polystyrene.
The polystyrene has an aminomethyl linker attached
to its surface.
The support coupling efficiency is about 98%.
The Cell Cycle NOTES
Overview of Cell Division
The ability of organisms to reproduce their kind is the one characteristic that best distinguishes living
things from nonliving matter.
The continuity of life is based on the reproduction of cells, or cell di
Title: Factors Affecting Enzyme Activity
Purpose: To determine the effects of temperature, pH, and substrate concentration on enzyme
Background: See Chapter 6 (Metabolism) in Campbell Biology (6 ed) or BioFact Sheet # 43
Question.1. provide energetics of complete oxidation of Palmitic acid and Palmitoleic
(a). Energetics of complete oxidation of Palmitic acid
Beta oxidation (7 Cyc)
Phospholipid lateral motion in a membrane is characterized by a diffusion coefficient
(D) of about 110-8 cm2/sec. The distance (r) traveled in two dimensions (over the
surface of (or in the plane of) the membrane) in a period of time (t) is giv
Question.2. A bacterium is in the process of acclimating to a higher temperature
environment. How would you expect the fatty acid complement (types and relative
amounts of fatty acids) in the bacterial membrane to change in response to the higher
3. In enolase, replacement of the catalytically active K345 by the unusual amino acid shown
below leads to a 1000 fold decrease in the catalytic rate.
a ) What is the chemical role of K345?
b ) Would you expect the side chain of the unusual a
Question.3. What is the energetic price, in terms of ATP equivalents, of breaking down
myristic acid (C14:0) to acetyl~CoA and then resynthesizing it?
Bioenergetics for the oxidation of Myristic acid
As Myristic acid has 14 carbons, no: of Acetyl C
3. Is -oxidation of fatty acids an aerobic or anaerobic pathway? Explain.
-oxidation is the oxidation of fatty acids at the beta carbon. It occurs within the
mitochondria and the end products of this pathway are Acetyl coA, NADH and FA
Part II. Please answer five (5) of the following questions 2-7. [Ten points
2. During glycogen degradation (reaction below), the splitting of glucose molecule of
of glycogen is spontaneous. During glycogen synthesis (reaction below), th
Below is a list of seven 2 carbon compounds (except CO2 which was doubled to make 2
carbons). Arrange these compounds in order of increasing oxidation level (i.e. from
most reduced to most oxidized).
CH3COH HOOC-COOH CH3COOH
CH2=CH2 CH3-CH3 CH3