April 8th 2009
Overview of the presentation
General facts on protein folding
Causes of misfolding and protein aggregation
Cellular consequences of protein aggregation
amino acids from the amino terminus
An activator is a substance, other than the catalyst or one of the
substrates, that increases the rate of a catalysed reaction. An activator
of an enzyme-catalysed reaction may he called an enzyme activator if
it acts by binding to the enzyme.
Properties of Enzymes
Enzymes as proteins are soluble in water or dilute salt
(b) Molecular weight
Enzymes have Mw (varying from 10000 - several
(c) Enzymes are charged molecules:
Due to the presences of amino acids, eac
HPLC is a popular method of analysis because it is easy to learn and use and is not limited by
the volatility or stability of the sample compound.
In isocratic HPLC the analyte is forced through a column of the stationary phase (usually a
enzyme engineering or enzyme technology the branch of biomolecular engineering concerned with processes designed to
produce, isolate, purify, and immobilize enzymes and to use
them for the catalysis of specific chemical reactions.
Enzymes are biocatalysts
Uses of Enzymes
Uses of enzymes can be classified as:(1)Medicinal and Clinical Use:
Enzymes can be used for Aiding Digestion. Example: Amylases, Proteases and
They can also be used as Deworming agents. Example: Papain.
Enzymes act as anti-clotting
An inhibitor is a substance that diminishes the rate of a chemical
reaction and the process is called inhibition. In enzyme-catalysed
reactions an inhibitor frequently acts by binding to the enzyme, in
which case it may be called an enzyme inhibitor. An a