Enzymes 3 Objectives:
1. Know the basic definition of an inhibitor and the three
types of inhibition we discussed in class.
2. Be able to define the types of inhibitor by identifying
which enzyme species interact with the inhibitor.
3. Recognize and be ab
G715 EXAM 1
(10 points; Avg = 4.3; Range = 1-9)
1. Provided below is a sequence alignment obtained for the RNase H domains of MMLV and
HIV-1 reverse transcriptases using PSI-BLAST. (MMLV- Moloney murine leukemia virus; HIV-1- human
G715 Exam 2 Ave 73 std dev -/+ 8.5
1. Part A:
Gilligan has cloned a new protein. The Professor had him generate a hydropathy plot,
shown below. Gilligan proposes that the protein has 10 transmembrane domains.
MaryAnn, a new post-doc on the island,
G715 EXAM 4 12/5/08 (Average = 73.7 +/- 12.8)
Average = 7.1
1. (8 pts) Draw out the pathway for epinephrine synthesis from phenylalanine
(structures and enzyme names not required). Explain why tyrosine is a conditionally
essential amino acid for neurotran
G715 Exam 2
(Average = 6.6; range = 2-8)
1. (8 points) a. State the 3 laws (or principles) of thermodynamics.
The First Principle: Energy is conserved in the universe, but it may change its form.
The Second Principle: Any spontaneous process incr
G715 Exam 3
December 3, 2007
(10 points) (7.2 average)
1. For the steady state conversion of glucose to pyruvate, which three steps (give either
enzyme name or reaction eg Pyruvate Lactate) would you expect to have control
strengths adding up to one? How
Introduction to Proteomics
Description of Proteomics
Reasons to do Proteomics
Basic Techniques of Protein separation and visualization
What is Proteomics?
A Proteome comprises - the full set of proteins that make up
Objectives for Bioenergetics
1) Define the terms equilibrium and steady-state in relationship to the
concentrations of metabolic intermediates.
2) Define the terms metabolism, anabolism and catabolism.
3) How is cellular energy utilized to drive processes
A. Signal Transduction is the process by which
extracellular signaling molecules influence
intracellular signaling pathways to regulate the
activity of a cell
B. Potential Advantages of Signal Transduction:
1. Diversification of signal effect
1. Be able to derive the Michaelis-Menton equation.
2. Know the assumptions for the steady-state condition.
3. What are the conditions that underly the Briggs-Haldane relationship versus
those for the Michaelis-Menton equation.
1. Differentiate between the free energy of a reaction (G) and the free energy
of activation (G). Explain how enzymes alter the rates of reactions without
affecting the equilibrium of the reaction.
2. Know the relationship between G