GENERAL MICROBIOLOGY I
1. Explain in scope of microbiology.
Microbiology is the study of microorganisms (germs, viruses, microbes) too
small to see with the naked eye. Microbiologists study cell stru
Chapter 13: Microbe Human Interactions
Video: Microbes and Human Disease
Microbe and Human Interactions
A. Beneficial relationships
B. Disease causing relationships
1. Factors involved in giving humans the edge in the figh
CHAPTER 1 THE MAIN THEMES OF MICROBIOLOGY
Microbiology is introduced and the scope of this science is discussed. Microorganisms
and their impact on Earth, as well as on humans, are described. The origin and evolution
of prokaryotes and eukaryotes, as well
LAB 10 DNA EXTRACTION
1. Toasted wheat germ does not work for this lab. Explain why.
a. When you toast the wheat germ it is heated up too much and it will
denature the proteins, which means the DNA would be destroyed.
2. The temperature of the DNA solutio
PRELAB 13 EPIDEMIOLOGY LAB
1. Define epidemiology, epidemic, reservoir and carrier.
a. Epidemiology is the study of disease and the course of that disease in
b. An epidemic is when there is a large number of individuals in a
specific area tha
PRELAB 4 SPECIAL STAINS
1. List two kinds of bacteria that produce endospores. How are they different
from one another?
Bacillus and Clostridium are the two most common endospore forming bacteria.
Bacillus is aerobic, while Clostridium is anaerobic.
PRELAB 3 QUESTIONS SMEARS AND STAINS
1. What is the purpose of a smear?
A smear is used to adhere bacteria to a slide so it can later be stained without
just washing off the slide.
2. What is the main purpose of the simple stain?
The main purpose of a sim
PRELAB 9 GRAM POSITIVE UNKNOWN LAB
1. What are the possible genus names of the unknown microbes in this lab and
what do these genus names mean in terms of cellular shape and
The two possible genus names that we will encounter during this lab
PRELAB 6 VIRUS LAB
1. What was the purpose or goal of the Hershey-Chase Experiment?
They wanted to prove that DNA was the material that passed on genetic traits
and not protein.
2. How were they able to distinguish DNA from protein?
Hershey and Chase were
PRELAB 11 ANTIMICROBIAL AGENTS
1. What is the Kirby-Bauer disk diffusion method?
a. The Kirby-Bauer method uses Mueller-Hinton agar, which we streak
with our sample and then we place various antibiotic discs onto the
streaked areas and measure cellular de
PRELAB 12 CHEMICAL AND PHYSICAL METHODS OF MICROBIAL CONTROL
1. What is the difference between an antiseptic and disinfectant?
a. A disinfectant is a substance used to kill microbial growth on
nonliving surfaces such as a toilet.
b. An antiseptic is a sub
PRELAB 5- SERIAL DILUTIONS, POUR PLATES AND SPREAD PLATES
1. Why do microbiologists have to perform serial dilutions on a sample?
Serial dilutions are necessary to reduce the concentration of microorganisms in a
solution so that when it is cultured you ca
PRELAB 7 GROWTH CONDITIONS LAB
1. What are mesophiles, psychrophiles and thermophiles?
Mesophiles are mid-range temperature loving microbes. Psychrophiles are cold
loving microbes and thermophiles are heat loving microbes.
2. How does temperature enzymati
Experiment 1: Tonicity and the Animal Cell
In this experiment you will explore the effects of osmosis on eggs. An egg is surrounded
by a permeable membrane and a hard shell. To observe the effects of osmosis the shell
will first need to be removed.