Gen511: Monday 10 March 2008
Chapter 14: Splicing Genetic analysis of U2-branchpoint interaction Association of snRNPs with pre-mRNA Splicing protein interactions: a. Synthetic lethal screens b. yeast 2-hybrid Role of RNA PolII CTD How are multiple i
Gen511: Wednesday 12 March 2008
Chapter 14: More on Splicing!
Role of RNA PolII CTD How are multiple introns spliced? Alternative splicing Self-splicing introns Group I introns Group II introns
Role of the RNA Polymerase II CTD
C-terminal domain o
Chapter 15 Messenger RNA Processing II:
Capping and Polyadenylation
Cap: G residue and methylations added to 5' end of eukaryotic mRNAs.
Polyadenylation: String of A residues (~250) added to the 3' end of mRNA.
Why the Cap and polyA?