Lecture 3, Sept 10 2012 Cell structure and function
Bacterial cell in general
- A bacterial cell consists of cytoplasm surrounded by an envelope.
o The cytoplasm contains DNA in a nucleoid (for prokaryotes).
Eukaryotes have a nuclear envelope versus a nu
Lecture 15, Oct 10 2012 Chapter 13, Organotrophic fueling
- Bacteria have tmRNA.
- Peptide becomes tagged with tag sequence to form SsrA-tagged peptide.
o Ssp then binds to the SsrA-tagged peptide for garbage disposable through ClpXP
Lecture 14, Oct 5 2012 Chapter 10, DNA replication and protein transcription, and
How do bacteria sense/monitor faulty structures and how do they respond?
- EX: SOS response.
o The SOS response stops DNA synthesis but does not stop biosynthesi
Lecture 13, Oct 3 2012 Chapter 10, Metabolism and genome evolution
Type IV pili
- Type IV pili are involved in twitching motility on solid surfaces.
o EX: Pseudomonas aeruginosa which can grow as biofilms on the lungs (cystic
fibrosis), burns, and hospita
Lecture 12, Oct 1 2012 Chapter 9 and 10, Recombination and molecular regulation
E. coli F pili
- Cells carrying F plasmids not only are capable of conjugation but also for forming
complex mature biofilms.
- F pilus connections between cells activate the p
Lecture 11, Sept 28 2012 Chapter 9, Gene transfer, mutations, and genome evolution
- Name the bacterium on the cover of the 1st edition textbook. Describe
the 3 fluorescent signals shown. What is being revealed by the
fluorescence and how
Lecture 10, Sept 26 2012 Molecular basis of bacterial pathogenicity (by Prof. Brett
Quiz question: write 6 articulate sentences about the differences and functions the four receptors
involved in the chemotaxis machinery.
Lecture 9, Sept 24 2012 Chapter 5, Bacterial motility chemotaxis
Basic mechanisms of motility
- Swimming is done by bacteria in a liquid environment that has low viscosity.
o This is done through external flagella.
Flagella are anchored in the cell membr
Lecture 8, Sept 21 2012 Chapter 4, Transport systems
Note: think about binding, translocation, and molecular mechanism behind release.
- Group translocation is done by a family of proteins that use phosphate derived energy
from PEP to
Lecture 7, Sept 19 2012 Chapter 4, Bacterial growth transport systems
- Cellular membranes are composed of a lipid bilayer (double layer of phospholipids with
heads facing outwards).
o They separate the cytoplasm from the periplasm.
Lecture 6, Sept 17 2012 lactam antibiotics
- They have different properties to inhibit different microbes.
- They can diffuse across the outer membrane of Gram-negative bacteria or through porins.
- lactamase cleaves the lactam ring to
Lecture 5, Sept 14 2012 Chapter 3, Peptidoglycan
Note: Quiz is postponed until Wednesday (covers up to only this lecture).
Homework: look at Inside a living cell by David Goodsell
- Cut out figure 1a, 1c, and 1d
o Trim right to edge = no border
o Write na
Lecture 4, Sept 12 2012 Chapter 3, Cell structure and function
How to study the cell
- Genetic analysis is used to show the biological function of different genes.
o This could be through observation of a phenotype after knockout of a gene.
This may affe