DNA, RNA, etc. objectives Part 1
Learning objectives: Human genome
1. The central Dogma says that DNA contains information that codes for proteins, yet only
1.5% of human DNA serves direct coding functions. Explain why this is true and what
the rest of ou
DNA, RNA, etc. objectives Part 3
Learning objectives: Protein synthesis
1. Describe the two step tRNA charging reaction. How is fidelity maintained and why is that
Step 1 = an aminoacyl-tRNA synthetase (which will be unique to each amino acid)
DNA, RNA, etc. objectives Part 2
Learning Objectives: Transcriptional Regulation
1. Describe the role of lactose and glucose levels in regulating the lac operon. What happens
when lactose is present but glucose is high and when lactose is present and gluc
DNA, RNA, etc. objectives Part 4
Learning objectives: DNA replication
1. Describe in general terms the five basic features of DNA replication.
Semi-conservative (means that one parent strand is integrated into each new copy)
Semi-discontinuous (using Ok
DNA, RNA, etc. objectives Part 5
Learning objectives: Molecular Biology and Medicine
1. Describe in general terms the materials (reagents) that are used in Molecular Biology
technology. Where do the reagents come from and what types of things do they do?
Why do we have DNA Replication?
-For cell division, and therefore, cell proliferation.
What is the basic reaction of DNA Replication?
-5 deoxynucleoside triphosphate to the 3OH end of the growing chain
-Release of Ppi to
Purine and Pyrimidine Metabolism
Purines and pyrimidines: building blocks of RNA, DNA
- also carriers of energy (ATP), reducing equivalents (NADH/NADPH), e- (FAD),
metabolic intermediates (CoA), and methyl groups (SAM)
Two Synthetic Pathways:
1. de novo m