PARASITOLOGY LAB EXERCISES
University of Nebraska-Lincoln
John Janovy, Jr.
Copyright 2013 by John Janovy, Jr.
HOMEWORK #6 KEY
1. Identify the protein band(s) on the gel which are most likely to be responsible for the
enzyme activity? (You can give approximate sizes or indicate directly on gel with
arrows, asterisks, circles, etc.) Explain your answer.
A SOLUTION TO THE SPECTROPHOTOMETRY HOMEWORK PROBLEM
1. Preliminary examination of the 5 l and 50 l samples from both GEX and GM2
suggests that something in the bacteria preparation is inhibiting or interfering with the
assay. (The activity in the 50 l sa
Key to Homework #3
1. The first step is to calculate the concentrations of the components:
[NaCl] = 5.8 g 0.1 liter 58.44 g/mole = 1 M
total [MES] = 9.75 g 0.1 liter 195 g/mole = 0.5 M
[NaOH] = (3.5 ml)(1 M)/100 ml = 0.035 M
2. MES is a weak acid and the
Key to Homework 2
Calculation of number of macrophages bound.
o From the graph estimate the cpm and rfu for all of the samples (see figure
and cpm/rfu column in table)
o Use the 0 time point and subtract from the other values (see blank column
Homework #7 Key
The basic questions is why are there 2 proteins detected in the non-denaturing gels when only a
single protein/polypeptide is detected in SDS gels. The similarity in size between the protein
detected in SDS gels and the faster migrating (i
HOMEWORK #11 KEY
1. All 3 clones overlap and are likely part of the same gene (see Appendix 1 for alignment). The
order from 5'-3' is clone_z, clone_y, and clone_x. Appendix 2 shows how the 3 sequences
can be compiled into a single sequence.
2. There is o
Homework #9 Key
There are several approaches to this assignment. Below one possible approach is discussed.
You have isolated DNA from your favorite organism and digest 10 l of the DNA with EcoRI
(GAATTC). A 1:50 dilution of the DNA has an A260 of 0.13.
HOMEWORK #8 KEY
Draw a restriction map of the 20 kb DNA fragment. In other words, make a drawing
representing the virus genome which shows the relative positions (ie, order) of the restriction
sites. Either include a scale (in kb) or indicate the appro