Contractile Vacuoles in Microorganisms
Contractile vacuoles absorb excess water and wastes from a microorganism's cell and excrete them into the environment by contracting.
Describe the process of handling wastes in microorganisms
- Contractile vacuoles protect a cell from absorbing too much water and potentially exploding by excreting excess water.
- Wastes, such as ammonia, are soluble in water; they are excreted from the cell along with excess water by the contractile vacuoles.
- Contractile vacuoles function in a periodic cycle by expanding while collecting water and contracting to release the water.
- contractile vacuole: a vacuole that removes waste or excess water
- osmoregulation: the homeostatic regulation of osmotic pressure in the body in order to maintain a constant water content
- osmolarity: The osmotic concentration of a solution, normally expressed as osmoles of solute per litre of solution.
- hypertonic: having a greater osmotic pressure than another
Contractile Vacuoles in Microorganisms
A contractile vacuole (CV) is an organelle, or sub-cellular structure, that is involved in osmoregulation and waste removal. Previously, a CV was known as a pulsatile or pulsating vacuole. CVs should not be confused with vacuoles which store food or water. A CV is found predominantly in protists and in unicellular algae. In freshwater environments, the concentration of solutes inside the cell is higher than outside the cell. Under these conditions, water flows from the environment into the cell by osmosis. Thus, the CV acts as a protective mechanism against cellular expansion (and possibly explosion) from too much water; it expels excess water from the cell by contracting. However, not all species that possess a CV are freshwater organisms; some marine and soil microorganisms also have a CV. The CV is predominant in species that do not have a cell wall, but there are exceptions. Through the process of evolution, the CV was mostly eliminated in multicellular organisms; however it still exists in the unicellular stage of several multicellular fungi and in several types of cells in sponges, including amoebocytes, pinacocytes, and choanocytes.
Contractile vacuole of Euglena: Structure of Euglena: 1 - Flagellum; 2 - Eye spot / Pigment spot / Stigma; 3 - Photoreceptor; 4 - Short second flagellum; 5 - Reservoir; 6 - Basal body; 7 - Contractile vacuole; 8 - Paramylon granule; 9 - Chloroplasts; 10 - Nucleus; 11 - Nucleolus; 12 - Pellicle
The CV's phases of collecting water (expansion) and expelling water (contraction) are periodical. One cycle takes several seconds, depending on the species and the environment's osmolarity. The stage in which water flows into the CV is called diastole. The contraction of the CV and the expulsion of water from the cell is called systole. Water always flows from outside the cell into the cytoplasm; and only then from the cytoplasm into the CV. Species that possess a CV always use it, even in very hypertonic (high concentration of solutes) environments, since the cell tends to adjust its cytoplasm to become even more hyperosmotic (hypertonic) than the environment. The amount of water expelled from the cell and the rate of contraction are related to the osmolarity of the environment. In hyperosmotic environments, less water will be expelled and the contraction cycle will be longer.
The number of CVs per cell varies, depending on the species. Amoeba have one; Dictyostelium discoideum
, Paramecium aurelia,
and Chlamydomonas reinhardtii
have two; and giant amoeba, such as Chaos carolinensis
, have many. In some unicellular eukaryotic organisms (e.g., amoeba), cellular wastes, such as ammonia and excess water, are excreted by exocytosis as the contractile vacuoles merge with the cell membrane, expelling wastes into the environment. In Paramecium
, which, presumably, has the most-complex and highly-evolved CV, the vacuole is surrounded by several canals, which absorb water by osmosis from the cytoplasm. After the canals fill with water, it is pumped into the vacuole. When the vacuole is full, it expels the water through a pore in the cytoplasm that can be opened and closed.
Flame Cells of Planaria and Nephridia of Worms
Flame cells and nephridia remove the waste from bodies through filtration in a manner similar to a kidney.
Compare and contrast the way in which planaria and annelids handle waste products
- Nephridia are more evolved than flame cells because they can reabsorb useful metabolites before excretion of waste.
- Both nephridia and flame cells are ciliated tubules that filter fluids in the cell to remove waste.
- Flame cells are connected to a duct system of pores to expel wastes, while nephridia often are open to the exterior of the organism.
- flame cell: a specialized excretory cell found in the simplest freshwater invertebrates
- nephridium: a tubular excretory organ in some invertebrates
- nephridiopore: the external opening of a nephridium, where waste is excreted from the cell
- nephrostome: the funnel-shaped opening of a nephridium into the body cavity
Flame Cells & Nephridia
As multi-cellular systems evolved to have organ systems that divided the metabolic needs of the body, individual organs evolved to perform the excretory function. Excretory cells known as flame cells developed in flatworms, while nephridia developed as excretory cells in annelids.
Flame Cells of Planaria
Planaria are flatworms that live in fresh water. Their excretory system consists of two tubules connected to a highly-branched duct system that leads to pores located all along the sides of the body. The filtrate is secreted through these pores. The cells in the tubules are called flame cells (or protonephridia) because they have a cluster of cilia that looks like a flickering flame when viewed under the microscope. Flame cells function like a kidney, removing waste materials through filtration. The cilia propel waste matter down the tubules and out of the body through excretory pores that open on the body surface; cilia also draw water from the interstitial fluid, allowing for filtration. After excretion, any useful metabolites are reabsorbed by the cell. Flame cells are found in freshwater invertebrates, such as flatworms, including parasitic tapeworms and free-living planaria.
Flame cells and nephridia: In the excretory system of the (a) planaria, cilia of flame cells propel waste through a tubule formed by a tube cell. In (b) annelids, nephridia filter fluid from the body cavity.
Nephridia of Worms
Earthworms (annelids) and some other invertebrates, such as arthropods and mollusks, have slightly-more-evolved excretory structures called nephridia. A pair of nephridia is present on each segment of the earthworm. They are similar to flame cells in that they have tubules with cilia and function like a kidney to remove wastes, but they often open to the exterior of the organism. The ciliated tubules filter fluid from the body cavity and carry waste, including excess ions, through openings called nephrostomes. From the nephrostomes, excretion occurs through a pore called the nephridiopore. A nephridium is more evolved than a flame cell in that it has a system for reabsorption of some useful waste products, such as metabolites and ions, by a capillary network before excretion (unlike planaria that can only reabsorb useful metabolites after excretion).
Malpighian Tubules of Insects
Malpighian tubules remove wastes from insects by producing urine and solid nitrogenous waste, which are then excreted from the body.
Explain how insects use malpighian tubules to excrete wastes and maintain osmotic balance
- Malpighian tubules are found in the posterior regions of insects, where they work with glands in the rectum to excrete waste and maintain osmotic balance.
- Ions are transported through active pumps found in the malpighian tubules; as the ions are secreted, water and waste are drawn to the tubules due to the change in osmotic pressure.
- Nitrogenous wastes, such as uric acid, are precipitated as thick pastes or powder to be excreted.
- malpighian tubule: a tubule that extends from the alimentary canal to the exterior of the organism, excreting water and wastes in the form of solid nitrogenous compounds
- uric acid: a bicyclic heterocyclic phenolic compound, formed in the body by the metabolism of protein and excreted in the urine
- hemolymph: a circulating fluid in the bodies of some invertebrates that is the equivalent of blood
Malpighian Tubules of Insects
Malpighian tubules line the gut of some species of arthropods, such as bees. They are usually found in pairs in the posterior regions of arthropod alimentary canals; the number of tubules varies with the species of insect. The system of malpighian tubules consists of branching tubules, which increase their surface area, near the hemolymph (a mixture of blood and interstitial fluid that is found in insects, other arthropods, and most mollusks) and fat tissues. They are lined with microvilli for reabsorption and maintenance of osmotic balance. They contain actin for support.
Malpighian tubules work cooperatively with specialized glands in the wall of the rectum. Body fluids are not filtered, as in the case of nephridia. Instead, urine is produced by tubular secretion mechanisms by the cells lining the malpighian tubules that are bathed in hemolymph. Metabolic wastes, such as urea and amino acids, freely diffuse into the tubules, while ions are transported through active pump mechanisms. There are exchange pumps lining the tubules which actively transport H+
ions into the cell and K+
ions out; water passively follows to form urine. The secretion of ions alters the osmotic pressure, which draws water, electrolytes, and nitrogenous waste (uric acid) into the tubules. Water and electrolytes are reabsorbed when these organisms are faced with low-water environments and uric acid is precipitated and excreted as a thick paste or powder. By not dissolving wastes in water, these organisms are able to conserve water; this is especially important for life in dry environments.
Malpighian tubules in bees: Malpighian tubules of insects and other terrestrial arthropods remove nitrogenous wastes and other solutes from the hemolymph.
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