Nitrogen Fixation: Root and Bacteria Interactions
Plants cannot extract the necessary nitrogen from soil, so they form symbiotic relationships with rhizobia that can fix it as ammonia.
Explain the process and importance of nitrogen fixation
- Diatomic nitrogen is abundant in the atmosphere and soil, but plants are unable to use it because they do not have the necessary enzyme, nitrogenase, to convert it into a form that they can use to make proteins.
- Soil bacteria, or rhizobia, are able to perform biological nitrogen fixation in which atmospheric nitrogen gas (N2) is converted into the ammonia (NH3) that plants are able to use to synthesize proteins.
- Both the plants and the bacteria benefit from the process of nitrogen fixation; the plant obtains the nitrogen it needs to synthesize proteins, while the bacteria obtain carbon from the plant and a secure environment to inhabit within the plant roots.
- rhizobia: any of various bacteria, of the genus Rhizobium, that form nodules on the roots of legumes and fix nitrogen
- nitrogen fixation: the conversion of atmospheric nitrogen into ammonia and organic derivatives, by natural means, especially by microorganisms in the soil, into a form that can be assimilated by plants
- nodule: structures that occur on the roots of plants that associate with symbiotic nitrogen-fixing bacteria
Nitrogen Fixation: Root and Bacteria Interactions
Nitrogen is an important macronutrient because it is part of nucleic acids and proteins. Atmospheric nitrogen, which is the diatomic molecule N2,
or dinitrogen, is the largest pool of nitrogen in terrestrial ecosystems. However, plants cannot take advantage of this nitrogen because they do not have the necessary enzymes to convert it into biologically useful forms. However, nitrogen can be "fixed." It can be converted to ammonia (NH3
) through biological, physical, or chemical processes. Biological nitrogen fixation (BNF), the conversion of atmospheric nitrogen (N2
) into ammonia (NH3
), is exclusively carried out by prokaryotes, such as soil bacteria or cyanobacteria. Biological processes contribute 65 percent of the nitrogen used in agriculture.
The most important source of BNF is the symbiotic interaction between soil bacteria and legume plants, including many crops important to humans. The NH3
resulting from fixation can be transported into plant tissue and incorporated into amino acids, which are then made into plant proteins. Some legume seeds, such as soybeans and peanuts, contain high levels of protein and are among the most important agricultural sources of protein in the world.
Diagram of the Nitrogen Cycle: Schematic representation of the nitrogen cycle. Abiotic nitrogen fixation has been omitted.
Nitrogen fixation in crops: Some common edible legumes, such as (a) peanuts, (b) beans, and (c) chickpeas, are able to interact symbiotically with soil bacteria that fix nitrogen.
Soil bacteria, collectively called rhizobia, symbiotically interact with legume roots to form specialized structures called nodules in which nitrogen fixation takes place. This process entails the reduction of atmospheric nitrogen to ammonia by means of the enzyme nitrogenase. Therefore, using rhizobia is a natural and environmentally-friendly way to fertilize plants as opposed to chemical fertilization that uses a non-renewable resource, such as natural gas. Through symbiotic nitrogen fixation, the plant benefits from using an endless source of nitrogen from the atmosphere. The process simultaneously contributes to soil fertility because the plant root system leaves behind some of the biologically-available nitrogen. As in any symbiosis, both organisms benefit from the interaction: the plant obtains ammonia and bacteria obtain carbon compounds generated through photosynthesis, as well as a protected niche in which to grow.
Rhizobia: Soybean roots contain (a) nitrogen-fixing nodules. Cells within the nodules are infected with Bradyrhyzobium japonicum, a rhizobia or "root-loving" bacterium. The bacteria are encased in (b) vesicles inside the cell, as can be seen in this transmission electron micrograph.
Mycorrhizae: The Symbiotic Relationship between Fungi and Roots
Many plants form associations called mycorrhizae with fungi that give them access to nutrients in the soil, protecting against disease and toxicities.
Describe the symbiotic relationship of mycorrhizae and plant roots
- Because nutrients are often depleted in the soil, most plants form symbiotic relationships called mycorrhizae with fungi that integrate into the plant's root.
- The relationship between plants and fungi is symbiotic because the plant obtains phosphate and other minerals through the fungus, while the fungus obtains sugars from the plant root.
- The long extensions of the fungus, called hyphae, help increase the surface area of the plant root system so that it can extend beyond the area of nutrient depletion.
- Ectomycorrhizae are a type of mycorrhizae that form a dense sheath around the plant roots, called a mantle, from which the hyphae grow; in endomycorrhizae, mycelium is embedded within the root tissue, as opposed to forming a sheath around it.
- In endomycorrhizae, mycelium is embedded within the root tissue, as opposed to forming a sheath around it; these are found in the roots of most terrestrial plants.
- mycorrhiza: a symbiotic association between a fungus and the roots of a vascular plant
- hypha: a long, branching, filamentous structure of a fungus that is the main mode of vegetative growth
- mycelium: the vegetative part of any fungus, consisting of a mass of branching, threadlike hyphae, often underground
Mycorrhizae: The Symbiotic Relationship between Fungi and Roots
A nutrient depletion zone can develop when there is rapid soil solution uptake, low nutrient concentration, low diffusion rate, or low soil moisture. These conditions are very common; therefore, most plants rely on fungi to facilitate the uptake of minerals from the soil. Mycorrhizae, known as root fungi, form symbiotic associations with plant roots. In these associations, the fungi are actually integrated into the physical structure of the root. The fungi colonize the living root tissue during active plant growth.
Through mycorrhization, the plant obtains phosphate and other minerals, such as zinc and copper, from the soil. The fungus obtains nutrients, such as sugars, from the plant root. Mycorrhizae help increase the surface area of the plant root system because hyphae, which are narrow, can spread beyond the nutrient depletion zone. Hyphae are long extensions of the fungus, which can grow into small soil pores that allow access to phosphorus otherwise unavailable to the plant. The beneficial effect on the plant is best observed in poor soils. The benefit to fungi is that they can obtain up to 20 percent of the total carbon accessed by plants. Mycorrhizae function as a physical barrier to pathogens. They also provides an induction of generalized host defense mechanisms, which sometimes involves the production of antibiotic compounds by the fungi. Fungi have also been found to have a protective role for plants rooted in soils with high metal concentrations, such as acidic and contaminated soils.
Mycorrhizae: Hyphae proliferate within the mycorrhizae, which appears as off-white fuzz in this image. These hyphae greatly increase the surface area of the plant root, allowing it to reach areas that are not depleted of nutrients.
There are two types of mycorrhizae: ectomycorrhizae and endomycorrhizae. Ectomycorrhizae form an extensive dense sheath around the roots, called a mantle. Hyphae from the fungi extend from the mantle into the soil, which increases the surface area for water and mineral absorption. This type of mycorrhizae is found in forest trees, especially conifers, birches, and oaks. Endomycorrhizae, also called arbuscular mycorrhizae, do not form a dense sheath over the root. Instead, the fungal mycelium is embedded within the root tissue. Endomycorrhizae are found in the roots of more than 80 percent of terrestrial plants.
Ectomycorrhizae: Ectomycorrhizae form sheaths, called a mantle, around the roots of plants, as shown in this image.
Nutrients from Other Sources
Many species of plants are unable to make their food via photosynthesis and must acquire nutrients in a variety of additional ways.
Differentiate among the sources of plant nutrition
- Some plants are parasites, which acquire all of some of their nutrients from another host plant and are, therefore, entirely dependent upon it for their survival.
- Saprophytes acquire nutrients from dead matter, using enzymes to convert complex organic compounds into simpler forms from which the plant can absorb nutrients.
- A symbiont experiences a mutually-beneficial arrangement with a plant; both partners contribute necessary nutrients to the other.
- An epiphyte is a plant that grows on other plants, but is not dependent upon the other plant for nutrition; instead, it uses the other plant for physical support.
- Insectivorous plants have special adapatations for attracting and trapping insects, which they use to supplement their own nutrients, depleted in the surrounding soil.
- photosynthate: any compound that is a product of photosynthesis
- photobiont: a photosynthetic symbiont
- mycobiont: the fungus that is a component of a lichen
- saprophyte: any organism that lives on dead organic matter, as certain fungi and bacteria
- epiphyte: a plant that grows on another, using it as a physical support but neither obtaining nutrients from it nor causing it any damage if also offering no benefit
- insectivorous: capable of trapping and absorbing insects; such as the sundew, pitcher plant and Venus flytrap
Nutrients from Other Sources
Some plants cannot produce their own food and must obtain their nutrition from outside sources. This may occur with plants that are parasitic or saprophytic: ingesting and utilizing dead matter as a food source. In other cases, plants may be mutualistic symbionts, epiphytes, or insectivorous.
A parasitic plant depends on its host for survival. Some parasitic plants have no leaves. An example of this is the dodder, which has a weak, cylindrical stem that coils around the host and forms suckers. From these suckers, cells invade the host stem and grow to connect with the vascular bundles of the host. The parasitic plant obtains water and nutrients through these connections. The plant is a total parasite (a holoparasite) because it is completely dependent on its host. Other parasitic plants, called hemiparasites, are fully photosynthetic and only use the host for water and minerals. There are about 4,100 species of parasitic plants.
Parasitic plants: The dodder is a holoparasite that penetrates the host's vascular tissue and diverts nutrients for its own growth. Note that the vines of the dodder, which has white flowers, are beige. The dodder has no chlorophyll and cannot produce its own food.
A saprophyte is a plant that does not have chlorophyll, obtaining its food from dead matter, similar to bacteria and fungi. (Note that fungi are often called saprophytes, which is incorrect, because fungi are not plants). Plants such as these use enzymes to convert organic food materials into simpler forms from which they can absorb nutrients. Most saprophytes do not directly digest dead matter. Instead, they parasitize mycorrhizae or other fungi that digest dead matter, ultimately obtaining photosynthate from a fungus that derived photosynthate from its host. Saprophytic plants are uncommon with only a few, described species.
Saprophytes: Saprophytes, like this Dutchmen's pipe (Monotropa hypopitys), obtain their food from dead matter and do not have chlorophyll.
A symbiont is a plant in a symbiotic relationship with other organisms, such as mycorrhizae (with fungi) or nodule formation. Root nodules occur on plant roots (primarily Fabaceae
) that associate with symbiotic, nitrogen-fixing bacteria. Under nitrogen-limiting conditions, capable plants form a symbiotic relationship with a host-specific strain of bacteria known as rhizobia. Within legume nodules, nitrogen gas from the atmosphere is converted into ammonia, which is then assimilated into amino acids (the building blocks of proteins), nucleotides (the building blocks of DNA and RNA, as well as the important energy molecule ATP), and other cellular constituents such as vitamins, flavones, and hormones.
Fungi also form symbiotic associations with cyanobacteria and green algae; the resulting symbiotic organism is called a lichen. Lichens can sometimes be seen as colorful growths on the surface of rocks and trees. The algal partner (phyco- or photobiont) makes food autotrophically, some of which it shares with the fungus; the fungal partner (mycobiont) absorbs water and minerals from the environment, which are made available to the green alga. If one partner was separated from the other, they would both die.
Symbionts: Lichens, which result from the symbiotic relationship between fungi and green algae, are often seen growing on trees.
An epiphyte is a plant that grows on other plants, but is not dependent upon the other plant for nutrition; it is non-parasitic. The epiphyte derives its moisture and nutrients from the air, rain, and sometimes from debris accumulating around it instead of from the structure to which it is fastened. Epiphytes have two types of roots: clinging aerial roots (which absorb nutrients from humus that accumulates in the crevices of trees) and aerial roots (which absorb moisture from the atmosphere).
An insectivorous plant has specialized leaves to attract and digest insects. The Venus flytrap is popularly known for its insectivorous mode of nutrition and has leaves that work as traps. The minerals it obtains from prey compensate for those lacking in the boggy (low pH) soil of its native North Carolina coastal plains. There are three sensitive hairs in the center of each half of each leaf. The edges of each leaf are covered with long spines. Nectar secreted by the plant attracts flies to the leaf. When a fly touches the sensory hairs, the leaf immediately closes. Fluids and enzymes then break down the prey and minerals are absorbed by the leaf. Since this plant is popular in the horticultural trade, it is threatened in its original habitat.
Insectivorous plants: A Venus flytrap has specialized leaves to trap insects, which it uses to supplement the low level of nutrients in the soil in which it lives.
Licenses and Attributions