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Microbiology: Laboratory Theory & Application, Brief 3rd Edition

Microbiology: Laboratory Theory & Application, Brief (3rd Edition)

Book Edition3rd Edition
Author(s)Leboffe, Pierce
ISBN9781617314773
PublisherMorton Publishing
SubjectBiology
Section 1-1: Glo Germ Hand Wash Education System
Observations and Interpretations
Section 1-2: A Comparison of Hand-Cleaning Agents
Observations and Interpretations
Section 1-3: Nutrient Broth and Nutrient Agar Preparation
Observations and Interpretations
Section 1-4: Common Aseptic Transfers and Inoculation Methods
Observations and Interpretations
Section 1-5: Streak Plate Method of Isolation
Section 1-6: Spread Plate Method of Isolation
Observations and Interpretations

Chapter 1, Section 1-1, Observations and Interpretations, Exercise 1

Page 19

Here is a tip:

Since the majority of people are right-handed, this hand is expected to be used more throughout the day. With frequent use comes more environmental exposure. 

Explanation

Before washing the left hand, it is expected to have more bacterial contamination than compared to after washing it. The same theory applies to the right hand.

  • Since most people are predominantly right-handed, the right hand is expected to demonstrate more bacterial contamination than the left hand (before and after hand washing). This is due to a more frequent use and exposure to more environmental objects.
  • Certain areas will not improve much after hand washing, since some surface areas are not flat and most people do not do an effective job of scrubbing them well enough.
  • These areas are deemed hard to reach and have hidden crevices for bacteria to harbor in, such as under the finger nails and in between the fingers.
  • These two areas will demonstrate the most bacterial contamination in general, even after hand washing, they will not become completely free of bacteria.

Sample Response

Before washing the left hand:

  • The fingers will be ++.
  • The palms will be ++.
  • Between the fingers will be +++.
  • Under the fingernails will be +++.
  • The tops of the fingernails will be +.
  • The front of the wrist will be +.
  • The back of the wrist will be +.
  • The back of the hand will be +.

 

After washing the left hand:

  • The fingers will be 0.
  • The palms will be 0.
  • Between the fingers will be +.
  • Under the fingernails will be ++.
  • The tops of the fingernails will be 0.
  • The front of the wrist will be 0.
  • The back of the wrist will be 0.
  • The back of the hand will be 0.

 

Before washing the right hand:

  • The fingers will be +++.
  • The palms will be +++.
  • Between the fingers will be +++.
  • Under the fingernails will be +++.
  • The tops of the fingernails will be ++.
  • The front of the wrist will be ++.
  • The back of the wrist will be +.
  • The back of the hand will be ++.

 

After washing the right hand:

  • The fingers will be +.
  • The palms will be +.
  • Between the fingers will be ++.
  • Under the fingernails will be +++.
  • The tops of the fingernails will be +.
  • The front of the wrist will be 0.
  • The back of the wrist will be 0.
  • The back of the hand will be 0.
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