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Inorganic phosphate labeled with 32P is mixed with glucose to a glycogen-free extract from liver, and the mixture is then incubated in the absence of...

Inorganic phosphate labeled with 32P is mixed with glucose to a glycogen-free extract from liver, and the mixture is then incubated in the absence of oxygen. After a short time, you isolate the 1,3 - bisphosphoglycerate from the mixture. Which carbons would you find radioactive phosphate? If you allow the incubation to continue for a longer period of time, will there be any change in the labeling pattern? Why or why not?

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Carbon 1 and 2 of 1,3-bisphosphoglycerate will... View the full answer

1 comment
  • Great, thank you Dr. Mariko. Why will there by a change in labelling pattern with continued incubation?
    • Dentistry11
    • Aug 06, 2018 at 3:32pm

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