Inorganic phosphate labeled with 32P is mixed with glucose to a glycogen-free extract from liver, and the mixture is then incubated in the absence of oxygen. After a short time, you isolate the 1,3 - bisphosphoglycerate from the mixture. Which carbons would you find radioactive phosphate? If you allow the incubation to continue for a longer period of time, will there be any change in the labeling pattern? Why or why not?
Carbon 1 and 2 of 1,3-bisphosphoglycerate will... View the full answer
- Great, thank you Dr. Mariko. Why will there by a change in labelling pattern with continued incubation?
- Aug 06, 2018 at 3:32pm