Sometimes there is an unknown amount of water in the chemical bottle so when you weigh the chemical some of the balance reading is water. Another way to accurately determine the concentration of the solution you just made is to measure the absorbance on a spectrophotometer and calculate the concentration from the absorbance and extinction coefficient. If the EM (molar extinction coefficient) is 34050 M-1cm-1at 280 nanometers wavelength for lysozyme, using Beer's law determine the predicted absorbance for your lysozyme solution you made above solution. Explain what may have happened if the absorbance measurement is lower than you predict.
Using Beer's law, absorbance for the lysozyme solution can be obtained... View the full answer