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500 0 10 - SOOO H Z ) ( # 4 ) ( 5 pts) The panels below show cross- sections through 3 D HINCA and HIN ( CO) CA triple- resonance NMR spectra of a...

Please help me out with this problem from my study guide. It is about analyzing 3D HNCA and HN(CO)CA triple resonance NMR spectra of a protein. IMG_0112.jpg

IMG_0112.jpg

500 0
10 - SOOO H Z )
( # 4 ) ( 5 pts) The panels below show cross- sections through 3 D HINCA and HIN ( CO) CA triple- resonance
NMR spectra of a protein ( panels # 1 and # 2 are 2D 1H - 13 C planes though the 3D data sets taken at
123 and 1 18 ppm in the nitrogen - 15 dimension ) . Each plane contains data from a single amino acid
in the protein . The amino acids are adjacent to one another in the primary sequence .
( a ) Assign the cross - peaks in the spectra below
and indicate how they are related to one another
Plane # 1
Plane #1
in the primary sequence . Label the cross - peaks .
HNICOICA 15 N = = 123 PPM
HINCA ISN = = 123 Rem
( b ) What type of amino acid most likely gives
rise to the data shown in plane # 1 ? How can
you tell ?
10
10*
( C ) Using the chemical shifts shown in the
Plane # 2
Plane # 2\
HNICOICA 15 N =_ 11.8 Ppin
HINCA ISN =_ 118 Ppin
HNCA and HIN ( CO ) CA spectra draw the 2D
IH - ISN HIS QC Spectrum that you would
expect to observe . Label the axes in the 20
spectrum .
IsA

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