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How do you solve this and find the net charge?


4. Explain the elution profiles of fluorescein and cytochrome C based on the pka and pl values. [Hint: you
should probably use the Henderson-Hasselbalch equation to see how much fluorescein is expected to
be in a protonated / deprotonated state at each pH value. For cytochrome C, you can use the excel
worksheet available on Canvas to calculate the net charge of the protein at pH 5 and 8) 1.5 pts

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