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4. (This one is a bit challenging! Attempt it if you're enjoying this kind of puzzle solving.)
Determine the primary structure of a polypeptide that generates the following data with
treatments as shown:
a) Acid hydrolysis: arg, asx, 2 cys, gly, ile, leu, lys, met, phe, pro, ser
b) One round of Edman's degradation: leu and ser
c) Carboxypeptidase A: asp
d)
DTT + iodoacetic acid followed by trypsin digestion: dipeptide made of arg and
ser, dipeptide made of asp and met, a hexapeptide made up of cys, gly, ile, leu,
phe, pro, and a dipeptide made of cys and lys.
e) DTT + 2-bromoethylamine followed by trypsin digestion renders a dipeptide
made of arg and ser, a dipeptide made of asp and met, free cys , free lys, and two
tripeptides, one cys, gly, and leu and the other ile, phe, and pro. (2-
bromoethylamine puts a + charge on cys, making it sensitive to trypsin digestion.
f) Chymotrypsin has no effect.
g) Pepsin gives a nonapeptide ( arg, asp, 2 cys, gly, leu, lys, met, ser) and a
tripeptide (ile, phe, pro)

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