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5. A denaturation/renaturation (similar to the one carried out by Anfinsen with ribonuclease)
experiment was carried out using insulin. However, in contrast to Anfinsen's results, only less than
10% of the activity of insulin was recovered when urea and B-mercaptoethanol were removed by
dialysis. (This is the level of activity you would expect if the disulfide bridges paired randomly.) In
contrast, if the experiment is repeated with proinsulin, full activity is restored upon renaturation.
Explain these observations.

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Proinsulin is the prohormone precursor to insulin made in the beta cells of the islets of Langerhans, specialized regions of... View the full answer

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