Did you know that the bananas that we eat today are totally different than the ones our grandparents ate? The Gros michel variety of banana is a plumper, sweeter, more flavorful fruit that contains large seeds. However, it was driven to near extinction in the 1950s by Panama disease, cause by the fungal pathogen Fusarium oxysporum. When that happened, banana farmers were forced to destroy their plants and switch to a different variety of banana called Cavendish, which is resistant to Manama disease. In reality, however, the cavendih is only resistant to the strain which killed off most of the Gros Michel variety. A new strain of Fusarium oxysporum called Tropical Race 4 (TR4) has begun to attack Cavendish plantations, resulting in a panic among the banana farming community. The spread of TR4 could deal severe economic losses to the entire industry yet again. Using Biotechnology design a transgenic plant that will be resistant to Fusarium oxysporum. Describe the various genetic features you need for proper expression, as well as any other considerations which need to be taken into accont. Describe the cloning strategy and all the methods you use as well as your chosen transfection method.
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