Let's say you have two tubes, 1 and 2, each carrying a bacterial sample. In one of the tubes the bacteria has been transformed with ONLY a vector and...
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Let's say you have two tubes, 1 and 2, each carrying a bacterial

sample. In one of the tubes the bacteria has been transformed with ONLY a vector and in the tube with a RECOMBINANT (vector +insert) construct. Using ONLY two techniques-PCR and gel electrophoresis (use both), how will you determine which tube carries a recombinant and which tube the vector alone?
Describe where the primers will anneal (not specific sequence but general location), what you expect to observe on the gel and how you will analyze and draw conclusions from the results? A schematic may be helpful!

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