Let's say you have two tubes, 1 and 2, each carrying a bacterial

sample. In one of the tubes the bacteria has been transformed with ONLY a vector and in the tube with a RECOMBINANT (vector +insert) construct. Using ONLY two techniques-PCR and gel electrophoresis (use both), how will you determine which tube carries a recombinant and which tube the vector alone?
Describe where the primers will anneal (not specific sequence but general location), what you expect to observe on the gel and how you will analyze and draw conclusions from the results? A schematic may be helpful!

Answer & Explanation
Verified Solved by verified expert

, dictum v

a. Fusce dui lectus, congue vel laoreet ac, dictum vitae odio. Donec aliquet. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Nam lacinia pulvinar tortor nec facilisis. Pellentesque dapibus efficitur laoreet. Nam risus ante, dapibus a molestie consequat, ultrices ac magna. Fusce dui lectus, congue vel laoreet

Unlock full access to Course Hero

Explore over 16 million step-by-step answers from our library

Subscribe to view answer
1 Attachment