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You want to determine whether the CD4+ T cell numbers in a blood sample from person exposed to HIV are normal or not. You have a flow cytometer and...

You want to determine whether the CD4+ T cell numbers in a blood sample from person exposed to HIV are normal or not. You have a flow cytometer and all the necessary tools, and the following reagents: goat IgG antibodies specific for human CD4, rabbit anti-goat IgG antibodies conjugated to the fluorochrome phycoerythrin, and chicken anti-goat IgM antibodies conjugated to fluorescein. What would be the best approach to use to analyze a blood sample from this person? 


a)use the fluorescein-conjugated chicken anti-goat IgM antibody and the goat anti-human CD4 antibody to carry out indirect immunofluorescence and measure CD4+ numbers by flow cytometry


b)use the anti-human CD4 antibody to carry out direct immunofluorescence and measure CD4+ cell numbers by flow cytometry


c)use the fluorescein-conjugated chicken anti-goat IgM and the phycoerythrin-conjugated rabbit anti-goat IgG to make a sandwich ELISA to quantify CD4+


d) use the goat anti-human CD4 and the phycoerythrin-conjugated rabbit anti-goat IgG to carry out indirect immunofluorescence and measure CD4+ cell numbers by flow cytometry


e) use the goat anti-human CD4 and the phycoerythrin-conjugated rabbit anti-goat IgG to carry out direct immunofluorescence and measure CD4+ cell numbers by flow cytometry

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