View the step-by-step solution to:

Pre- class activity Directions :` $ 1 . Go to the DNAi website www . dnai . org> Manipulation}} Techniques> sorting and sequencing. 2 . View

IMG_0085.jpgA. How does the process of gel electrophoresis separate DNA fragments?

B. What is the purpose of the agarose gel?

C. What is the purpose of adding blue "tracking" dye to the DNA samples?

D. Explain why DNA has an overall negative charge.

E. Why is the fact that DNA has a negative charge so important in the gel electrophoresis process?

F. Explain how an agarose gel can separate DNA fragments of different lengths.

G. What is the purpose of ethidium bromide in gel electrophoresis?

IMG_0086 (1).jpgH. Why is a marker used when running the fragments through the gel?

I. What is a restriction map?

J.  On the gel picture below, (a) circle the smallest fragment produced by a restriction enzyme and label it "smallest." (b) circle the largest fragment produced by a restriction enzyme and label it "largest."

K. In one or two sentences, summarize the technique of gel electrophoresis.

IMG_0087.jpgIMG_0088.jpgIMG_0089.jpg

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Pre- class activity
Directions :`
$ 1 . Go to the DNAi website www . dnai . org> Manipulation}} Techniques> sorting and sequencing.
2 . View the Gel Electrophoresis 2 - D animation , and answer the following questions.
Questions :"
1 . How does the process of gel electrophoresis separate DNA fragments ?"
2 . What is the purpose of the agarose gel ?"
3 . What is the purpose of adding blue " tracking " dye to the DNA samples ?
4 . Explain why DNA has an overall negative charge.
5 . Why is the fact that DNA has a negative charge so important in the gel electrophoresis process ?
6 . Explain how an agarose gel can separate DNA fragments of different lengths.
7 . What is the purpose of ethidium bromide in gel electrophoresis ?"

IMG_0086 (1).jpg

8 . Why is a marker used when running the fragments through the gel ?
9 . What is a restriction map ?
10 .
On the gel picture below.
( a ) circle the smallest fragment produced by a restriction enzyme and label it " smallest ."
( b ) circle the largest fragment produced by a restriction enzyme and label it " largest."
MARKER
BamHil
BamHI
LOOK!
ECORI
GEL PHOTO
11 . In one or two sentences , summarize the technique of gel electrophoresis.

IMG_0087.jpg

IMG_0088.jpg

Making a DNA fingerprint
In this activity , you will model the construction of DNA fingerprints for a viral genome using different
restriction enzymes . You will also practice interpreting restriction maps and visualize how the process of gel
electrophoresis separates DNA fragments
DNA restriction fragment size chart
Directions :"
List your DNA fragments in the following chart under the column of the appropriate restriction enzyme.
List each fragment , from largest to smallest
ECORI
Hind III
|BamHI
\NCOI
BMrI
STUI

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