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Procedure I - Group 1: Known Sample Solutions

 

Complete the table below using your data from Procedure I. For each sample solution (A-E) indicate any color change that occurs when a test solution is added. Fill in the last column (Biomolecule Classification) using data found under the Activity Form tab.

 

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Observations and Questions

 

[1] Develop a  hypothesis statement for the experiment you conducted for Procedure I.

 


 

Based on the independent variables in the test solutions reactions from data tables 1 and 2, how can one predict the dependent variables changing in the biomolecule classification results on data table 2?

 

 

 

 

[2] Which variable represents the independent variable? What information helped you to come to that decision?

 

 

 

The independent variable is represented in the test solution reaction using the base color patterns of red, blue, green, purple and gray. For example, if the row started with red and had no more of the base color patterns following it was found to be consistent with "sugar".

 

 

[3] Would you characterize this experiment as qualitative or quantitative? What information helped you to come to that decision?

 


 

I define this experiment to be quantitative by identifying the reoccurring color patterns in the test solution reaction column to be able to identify the appropriate biomolecule classification selection.

 

 

[4] Refer to your completed Data Table I and place the appropriate answer to each of the statements below.

 


 

 

Test Solution 1 reacts with: Sugar

Test Solution 2 reacts with: Starch

Test Solution 3 reacts with: Fat

Test Solution 4 reacts with: Protein

Test Solution 5 reacts with: Amino Acid


Procedure II - Group 2: Unknown Sample Solutions

 

Complete the table below using your data from Procedure II. For each sample solution (A-E) indicate any color change that occurs when a test solution is added. Next, use Data Table I to determine the biomolecule classification for each sample solution. Hint: compare the color changes in both tables to make your determinations.


 

 

Observations and Questions

 

[5] Did any of your unknown solutions contain reducing sugars? If yes, which one? What information helped you to come to that decision?

 

 


 

Yes, the solution of starch contained sugar because when I referenced the data table one the color red is the only independent variable that is consistent with the sugar biomolecule solution. Also, sugar contains starch.

 

 

 

[6] Did any of your unknown sample solutions contain protein? If yes, which one? What information helped you to come to that decision?

 


 

Yes, the solution to amino acid contained protein because when referenced the data table it has the color purple and protein contains amino acid in the molecule.

 

 

[7] Using the laboratory tests in this experiment, would you be able to distinguish between small molecules such as amino acid from a larger molecule such as a protein? Explain why or why not.

 

 


 

Yes, by identifying the columns that have more than one of the base colors (red, blue, green, purple and gray) are consistently a smaller molecule as it relates to data table one.

 

 

 

[8] Using the laboratory tests in this experiment, would you be able to distinguish between a starch such as a potato and a (reducing?) sugar such as glucose? Explain why or why not.

 


 

Yes, by identifying the columns that have more than one of the base colors (red, blue, green, purple and gray) would be starches such as potatoes but sugar would only have one molecule since it is a bigger molecule.

 

 

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