1.) During a fed-batch bioreactor run 1M NaOH (~1600 mM) is used to control the pH. In addition, a feed
solution with an osmolarity of 1500 mOsm is used for fedbatch operation. The starting cell culture medium has an osmolarity of 320 mOsm. If the total base addition was 30 mL/L of culture and if the total feed was 300 mL/L of the culture, estimate the final osmolarity.
2.) A microcarrier culture will be used for cell therapy applications. This process uses non-porous microcarriers in a 500L bioreactor. After the cells are expanded on the microcarriers, they use release from the microcarriers using trypsin. The following properties are provided for the microcarriers: Diameter= 160 um Particle Density= 1.08 g/L Specific area= 12000 cm2 /gram Max Capacity= 6000 Cells/cm2 If the culture uses 5 g/L of these microcarriers, how many cells can be produced in 500L bioreactor?
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