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  1. (20pts) If you observed growth of your transformed bacterial cells on LB/AGAR alone (no

antibiotics), the LB AGAR + KAN, and on the LB AGAR + AMP plates BUT NOT on the LB AGAR + AMP & KAN plate. 

 

                                              i.    (10pts) In particular, what would these results indicate about the DNA that was transformed into these bacterial cells?

 

1.   Explain your answer

 

 

                                             ii.    (10pts) Moreover, these results would MOST LIKELY indicate an error at which step(s): Digestion, Ligation, Transformation, and/or Plating?

 

1.   Explain your answer in

 

Top Answer

This means you have some bacteria with the pAMP plasmid and some with the pKAN plasmid, but none of the bacteria... View the full answer

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