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Procedure I - Group 1: Known Sample Solutions

 

Complete the table below using your data from

Procedure I. For each sample solution (A-E) indicate any color change that occurs when a test solution is added. Fill in the last column (Biomolecule Classification) using data found under the Activity Form tab.

 

Data Table I


 

Observations and Questions

 

[1] Write an hypothesis statement for the experiment you conducted for Procedure I.

 

A:

 

 

 

 

[2] Which variable represents the independent variable? What information helped you to come to that decision?

 

 

 

 

 

 

 

[3] Would you characterize this experiment as qualitative or quantitative? What information helped you to come to that decision?

 

 

 

 

 

 

 

[4] Refer to your completed Data Table I and place the appropriate answer to each of the statements below.

 

Answers: amino acid, starch, fat, sugar or starch, protein or amino acid

 

Statements:

 

Test Solution 1 reacts with: Sugar

Test Solution 2 reacts with: Starch          

Test Solution 3 reacts with: Fat    

Test Solution 4 reacts with: Protein

Test Solution 5 reacts with: Amino Acid

 

 

Procedure II - Group 2: Unknown Sample Solutions

 

Complete the table below using your data from Procedure II. For each sample solution (A-E) indicate any color change that occurs when a test solution is added. Next, use Data Table I to determine the biomolecule classification for each sample solution. Hint: compare the color changes in both tables to make your determinations.

 

 

Data Table II


 



 

 

Observations and Questions

 

[5] In Procedure II, did any of your unknown sample solutions (A, B, C, D, E) contain reducing sugars? If yes, which one? What information helped you to come to that decision?

 

 

 

 

 

 

[6] In Procedure II, did any of your unknown sample solutions (A, B, C, D, E) contain protein? If yes, which one? What information helped you to come to that decision?

 

 

 

 

 

 

 

[7] Using the laboratory tests in this experiment, would you be able to distinguish between a small molecule such as amino acid from a larger molecule such as a protein? Explain why or why not.

 

 

 

 

 

 

 

[8] Using the laboratory tests in this experiment, would you be able to distinguish between a starch and a sugar such as glucose? Explain why or why not.

 

 

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