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Subject:BiologyCourse:Principles of biology I On the left side of the figure below is a circle representing the purified plasmid, pGEN101.
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<br/><br/>Subject: BiologyCourse: Principles of biology I<br/><br/><br/><br/><br/>On

the left side of the figure below is a circle representing the purified plasmid, pGEN101.  Indicated on the figure are the sites where the plasmid is cut with BamHI and HinDIII separately (single digestion) and with both enzymes together (double digestion).  Also indicated on the figure is the size of the corresponding restriction sites.
On the right shows is an illustration of the stained electrophoresis gel following restriction enzyme digestion of the plasmid.


Which lane corresponds to the undigested plasmid?                             [ Select ]                          ["V", "I", "III", "IV", "II"]      


( Both lane I and V are wrong. The answer is lane II and I want to know why. Thanks)

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Subject: Biology, Science

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