Procedure: Cut two sticks of your hard vegetable to measure 0.5 cm x 0.5 cm x 4. Rinse the two vegetable sticks and pat them dry with paper towel....
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Water only 0.15 M sugar 0.3 M sugar 0.45 M sugar 0.6 M sugar 0 M sugar (1 teaspoon) (2 tsp) (3 tsp) (4 tsp)

Procedure: 1. Cut two sticks of your hard vegetable to measure 0.5 cm x 0.5 cm x 4.0 cm. Rinse the two vegetable sticks and pat them dry with paper towel. 2. Clean the small glasses or jars. Measure 100 mL of tap water into each glass. 3. In the first glass, you will only have water. This is a 0M solution. 4. In the remaining glasses. add the amount of sugar indicated in the table below. Stir to dissolve the sugar. Make sure you label the glasses or jars, so you know which concentration of solution is present in each one. 5. Add one vegetable stick into each glass. 6. Leave the vegetable sticks for 24 hours. '17'_Upm 7. Remove the vegetable sticks. Pat them dry with paper towel. Then, precisely measure their dimensions: length, width and height. Prepare solutions in each of the glasses as follows: ———m Glass 5 Water only 0.15 M sugar 0.3 M sugar 0.45 M sugar 0.6 M sugar 0 M sugar (1 teaspoon) (2 tsp) (3 tsp) (4 tsp) Interpreting the Results: Make a graph using data for 5 different sugar concentrations from your results. Draw a curve of best fit for these results. Interpret the pattern in your graphed results. Two good online graphing tools are Online Chart Tool, and Create- A-Graph. Evaluating the Procedure and Results: 1. Based on your observations, which of the glasses contained hypertonic solutions, and which were hypotonic? Explain how you know. 2. How would you know If any of the sugar solutions was isotonic to the cytoplasm inside the plant cells? Did any of the glasses contain an exact isotonic solution? 3. Explain, at the level of the cell, what was happening to make cells gain mass and lose mass. (consider the cell membrane, sugar molecules & their size, water molecules at their size)

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