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# BioE 440 / 640 Spring 2010 Homework #8 Due Tuesday, April 6, 2010 Based on Shuler (Chapter 11): Problem 1: A rotary drum filter with a filter area of...

Different Biochemistry Problems for Assignment

Problem 4: A new antibiotic is separated from a fermentation broth by adsorption on resin beads in a fixed bed. The bed is 5 cm in diameter and contains 0.75 cm^3 resin/cm bed. (Column height 50cm)

The overall mass transfer coefficient is 12h^-1 . If the antibiotic concentartion in the feed is 4 g/l and final concentration is 0.1g/L.

The equilibrium relationship is {C^*}S = 20({C^*}L )^1/2 , and the operating line relationship is CS = 5CL , where CS is g solute/l resin and CL is g solute/l solution.

determine the volumetric flow rate for the column (L/h)

(Book Problem: Bioprocess Engineering, Basic Concepts (2nd Edition); Shuler/ Kargi)

BioE 440 / 640 Spring 2010 Homework #8 Due Tuesday, April 6, 2010 Based on Shuler (Chapter 11): Problem 1: A rotary drum filter with a filter area of 200 m 2 and a vacuum pressure of 75 kPa is to be used to remove cells from a fermentation broth. Filter leaf tests have been performed on the fermentation broth. The following characteristics were determined: Viscosity ( μ ) = 5 cP Specific cake resistance ( ! ) = 1 x 10 11 cm/g Medium resistance (r m ) = 1 x 10 -8 cm -1 Cake solids per volume filtrate (C) = 15 g/L It is desired to operate the rotary drum filter with a cycle time of 45 seconds and a cake formation time of 10 seconds. 1) What is the filtration rate expected for this system? 2) How significant is the medium resistance? Problem 2: Determine the maximum flow rate for the centrifugation of a suspension of lysed bacteria cells by a plant-scale disk centrifuge . Disk/bowl centrifuge characteristics (see Table 5.5 on Centrifuge Handout) Bowl diameter = 25.4 cm Angle ( " ’) = 42° Distance to inside edge of disk (r 1 ) = 8 cm Distance to outside edge of disk (r 2 ) = 20 cm Number of disks (n’) = 100 In a laboratory centrifuge, it was determined that it took 17 minutes to clarify the lysed cells at 12,000 rpm. The laboratory centrifuge the top of the liquid was 32 mm and the packed solid was 79 mm from the center of rotation, respectively. Problem 3: Shuler problem 11.3 Problem 4: Shuler problem 11.4 (my version has a missing line, use these values) Column height 50 cm Final concentration 0.1 g/L Determine the volumetric flow rate for the column (L/h).
Problem 5: For the solute mixtures below, determine which could be separated by ion exchange chromatography, and describe the conditions and justify answers. 1. Glucose, D-malic acid, sodium sulfate 2. Lysine, glutamic acid 3. Acetic acid, glucose, lactic acid 4. Human serum albumin, sodium chloride, human IgG 5. Insulin, pro-insulin 6. Mannose, arabinose Based on Hall A. What was the solution used to isolate the plasmid DNA? B. What is the short name of the procedure now used to isolate plasmid DNA? C. Why was Genentech not required to use a P4 (BSL-4) facility, such was required for the Harvard group? D. What were the two reasons that Genentech needed Lilly for production and distribution of insulin? E. How much insulin was made in the first “batch” produced by the Genentech scientists? How does this compare to a single dose of insulin needed by a diabetic? From: Harrison, R. G. et al. (2003) Bioseparations science and engineering, Oxford Press, New York.
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Solution
Length = 50cm
k = 12/h
Cross sectional area (A)
= pi*r*r*h (r = d/2)
= pi*2.5*2.5*50 = 981.25cm2
Resins = 0.75 * 50
= 37.5 cm3 resins
We know , operating line
CS = 5CL
CS = 5 * 4 = 20g...

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