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In the "sandwich" analogy of the direct ELISA technique, what is the "bread"? What is the "filling"?

In the "sandwich" analogy of the direct ELISA technique, what is the "bread"? What is the "filling"?
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Solution:
In sandwich ELISA the filling can be defined as adding the Phosphate Buffered
Saline to the each well with buffer and removing it as a part of washing in the ELISA
methods. Bread can be...

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