I'm doing an experiment using a gene gun, and I need to answer a question that I'm stuck on. The question asks that starting with a 2.5 microgram/microliter solution of DNA, and 100 mg of M-10 tungsten particles, how would you prepare a series of 5 mg DNA per 1.2 mg particles solutions for transformation? How many total shots would you get from this preparation?
Am I supposed to set up 2.5ug/ul = 5mg/(x)ul and use this answer? I have attached my protocol if more information is necessary. I don't have any information on how to come about with this answer, so I am really very confused. Please help!
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