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Within the DNA sequence shown below, is a gene with restriction sites proximal to its start and stop codons. The 6 base restriction sites have...

Problem solving question on DNA sequence containing gene with restriction sites proximal to its start and stop codons. And the 6 base restriction sites have overhangs underlined respectively and those compatible with the listed cloning sites of the vector below Please see the data sheet attached.
Within the DNA sequence shown below, is a gene with restriction sites proximal to its start and stop codons . The 6 base restriction sites have overhangs (underlined ) respectively compatible with those listed as the cloning site of a vector. 1 TTAAAATGTA AATTATATTA AAATCGGTAT AATAGAAAGA GAGAATTTAA ATTGGGAATA 61 TACAAGTAAT ACAAAAATTA GGAGGAATTT CCC ATG CCTA TTAAAATTAA TCTTCCCAGC 121 ATGAAAAACA CCGCTGATAC ATGGTCGCTT GATGTTTTAC GGCATGGTAC TGTATACCGC 181 AATTTAAAAG ATAATAATTG TTATATTTAT CAAAATGAAC TAAACGGGCA TATTTATATA 241 ATTGAGTCCG GCGATTTTTA TTCTTTTGAA AATGAGTATG AAGCGAAAGA CCATTTACAG 301 GAAAATGATA TTCCTGACGT ATGGAAAGAA ACAAATCTCG CTTTTGTTGT AAGTCTTGAA 361 GCTAGCAAA T AG ATC TAAAT ATTAGTGTAT AGGAATATTC CTATACACTA ATAA VECTOR SITES: AatII (GACGT C), BglII (AGATC T), BspHI (TCATG A), MfeI (CAATT G), NdeI (CATA TG), NheI (GCTAG C), XbaI (TCTAG A). Question 2 (a). The start and the stop codons are showed in bold type : Note the sequence of the proximal restriction sites , referred to previously. And then WRITE out the sequence you have identified, one 6 letter sequence per line. What is the length of the gene? including flanking restriction sites, From the first base of the site by the start codon to the last base of the site by the stop codon? (Write Out This LENGTH). b). The gene and vector are ligated at their annealed restriction site overhangs. Of the listed enzymes in the VECTOR SITES above, decide if any could re-cleave each of the ligated ends: if so, name the enzyme(s) and explain why. And if not, then explain why not.
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c). Describe how you would select potential colonies from the transformation, and what laboratory methods you could use to determine whether these colonies have your gene cloned directly.
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Dear Student, Yes it is... View the full answer

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