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the specific activity of glycogen phosphorylase increases from 2.5U/mg homogenate protein to 325.5 U/mg protein after being bound to and eluted from...

the specific activity of glycogen phosphorylase increases from 2.5U/mg homogenate protein to 325.5 U/mg protein after being bound to and eluted from a cation exchange column at ph 2.7. which of the following is correct conclusion based on this information?
a. the yield of enzyme is greater than 80%
b. the enzyme is negatively charged at ph 2.7
c. the enzyme is purified over 100 fold
d. the enzyme is globular in structure
e. the enzyme is in an inactivated state

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