Final Applied Lab Project: The grade for the final applied lab project will be determined by the accuracy and completeness of the answers to the questions provided below.
Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. (Recall: Enzymes are proteins.) If necessary, it may be useful for you to review the Scientific Method Tutorial (found in the Course Content section of the classroom).
You may need the following, depending on your experimental design:
Materials in your lab kit:
Additional materials you may need:
• plastic beakers or cups
• hydrogen peroxide solution
• sample of fresh meat, about 1 cm cubed in size (unprocessed and uncooked, e.g., liver, steak, fish, or poultry—particularly organs)
• sample of fresh vegetable, about 1 cm cubed in size (e.g., potatoes or other root vegetables)
• a pen for labeling the beakers or cups
• a ruler with centimeter markings
1. What question are you asking? Clearly state your hypothesis.
2. Design an experiment. Provide a detailed account of the materials and methods used to conduct the experiment. Also include the methods for data collection and analysis.
3. Conduct the experiment and record your results below. What did you observe? Which samples showed bubbling?
4. Use your knowledge about enzymes to interpret your results. It may be necessary for you to refer to your textbook. What effect does the acid have on the enzyme?
5. Was your hypothesis supported? What is your conclusion?
If you reference any of the scientific literature in your write-up of the applied lab project, remember to cite those references using the APA format. Also, remember that you should answer the questions listed above with complete explanations and thus avoid using brief one- to two- sentence answers.
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