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BIO 100A Online Home Lab Reports Instructions and Grading Rubric (40p) Due by:59 PM PST on the second Sunday of class Directions 1. Before attempting...

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BIO 100A Online Home Lab Reports InstrucTons and Grading Rubric (40p) Due by: 11:59 PM PST on the second Sunday of class Direc±ons 1. Before a±empTng to perform a lab, read the lab's protocol in its enTrety, look over the relevant supplemental materials, and gather all of the necessary materials. 2. Exercise cau±on and respect the safety of yourself and others at all ±mes . 3. Keep notes as you perform experiments. 4. Use the lab reports to report your results. 5. Type your answers, observa±ons, and results in bold. 6. Save your report oFen as you ²ll it out, so as not to lose informaTon. 7. Use the "Save As" opTon to save your ²le as a Word ²le. 8. Save your lab report with this Fle name : Last name, underscore, ³irst IniTal, underscore H1. ´hus Charles Darwin would save his Unit 1 Home Lab Report 1 as Darwin_C_H1. 9. Submit reports 1-4 as under the corresponding assignment link in week 2 before 11:59 PM PST on the second Sunday of class. 10. Submit reports 5-8 as under the corresponding assignment link in week 4 before 11:59 PM PST on the second Sunday of class. 11. Please note that the reports will be scanned for plagiarism , and it will be µagged for both containing web content verbaTm and previously submi±ed papers- this includes your previous submissions if you have taken the class previously. Laboratory Reports Each of your lab reports MUS´ include the following components to receive full credit and be organized in the following way. 1. Purpose (4 pts): one to two sentences brieµy staTng the learning objecTve for the assigned lab. 2. Lab Summary (6 pts): Explain what was observed during the lab acTvity. ´his secTon should be approximately one paragraph in length. 3. Lab Answers (14 pts): Answers to the lab report quesTons that reµect and demonstrate your understanding on the concepts. (Should be wri±en in complete sentences for Labs 2-6) 1
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4. Discussion and Conclusions (16 pts): 1-2 paragraph learning refecton ThaT summarize The lab and speciFcally addresses The learning objectves relatng Them back To The daTa or observatons collecTed in The lab. 2
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Unit 1 Home Lab 1: Metric system Points: 40p Name: Purpose (4p) Lab Summary (6p) Lab Answers (14p) 1. Using a metric ruler, determine the length of the items in Table 1.1 below: In the final column, you are to estimate your measurement precision. To do this, measure each item a second or even third time. How close are the measurements? If there is a range of values for the length you measure, record the average difference between measurement values as your uncertainty. If your measured value for a given object appears the same after repeated measurements, this does not necessarily mean that your uncertainty is zero. Look closely at your ruler or measurement device and estimate the smallest unit of length that you would be able to discriminate with it. Every measurement device has limits. For instance, very few people use a ruler with a precision greater than 1/3 or 1/2 of a millimeter; in many cases, even this precision is difficult or impossible to obtain. Typically +/- 1 mm is standard for measuring flat objects with a ruler, but this uncertainty can be expected to go up when the object has significant curvature or its length is not quite so well defined. To measure the circumference (length around) of your head or thigh, wrap a piece of string around it and mark where the string meets itself. Then lay the string out flat and measure the length with your ruler. Updated May 2015
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Table 1.1. Metric measurements and uncertainties. meters cm mm inches Uncertainty Δ Your favorite shoe .254 25.4 254 10 2.54 Your index finger 0.0762 7.62 76.2 3 7.62 A pencil 0.1524 15.24 152.4 6 1.524 Fingernail of your pinky 0.0031 75 0.317 5 3.175 1/8 .3175 Width of a credit card The circumference of your thigh The circumference of your head 2. Measure and record volume in Table 1.2. Estimate the rough volume of your head by using the circumference (denoted C) and multiplying out this formula (based on the volume of a sphere =4 r 3 /3 = C 3 /(6  Volume 1/59 × C × C × C = C 3 /59 Estimate the uncertainty in your head volume (ΔV, called "delta V") calculation by using the uncertainty in your measurement of the circumference of your head (denoted ΔC) and multiplying through the following formula: ΔV 3/59 × C × C × ΔC = 3/59 × C 2 × ΔC Table 1.2. Head volume and uncertainty estimates. Circumference (C) Uncertainty in Circumference (ΔC) Head Volume ≈ 1/59 C 3 Uncertainty in Head Volume (ΔV) ≈ 3/59 × C 2 × ΔC 3. Complete the conversions in Table 1.3. The first row has been done. Table 1.3. Length conversions. Length km m miles feet 2.0 km 2.0 2,000 1.24 6,562 705 m 705 Updated May 2015
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Unit 1 Home Lab 2: Enzymes Points: 40p Name: Purpose (4p) Lab Summary (6p) Lab Answers (14p) 1. Fill in the following table. Compare all cups. Use relative terms to describe the size and number of bubbles in each cup. For instance, describe the Number of Bubbles using the terms: No bubbling, Moderate bubbling, Good bubbling, Very good bubbling. To describe average bubble size use the terms: Very small, Small, Large, or Very large . To describe pH without access to pH detectors, simply use the pH chart earlier in this chapter to describe each as acidic, neutral, or basic . To describe the Catalase Activity, use your data on the size and number of bubbles to estimate the amount of gas produced in the Catalase mediated process. Use the following terms: Very Low, Low, Moderate, High, Very high Table 2.1. Catalase reaction observations. Cup Number of Bubbles Size of Bubbles pH Catalase Activity 1 2 3 4 2. Bubbling indicates the formation of what chemical? Updated May 2015
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3. Describe the activity of Catalase as pH increases. Do you think that other enzymes are likely to behave in this way as well? Why or why not. 4. Assume that you have a pH meter which would enable you to very accurately measure the pH of a solution. Describe an experimental design that would allow you to pinpoint the exact pH at which Catalase is the most active. 5. Regarding cup #1: a) Describe the utility of cup #1 as a control. b) What other material did you introduce to this cup? Describe what you observed. How does Catalase activity in the material you investigated compare to potato? Discussion and Conclusions (16p) Updated May 2015
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Unit 1 Home Lab 3: Cellular RespiraTon Points: 40p Name: Purpose (4p) Lab Summary (6p) Lab Answers (14p) 1. List the following experimental materials: a) Kind of yeast used: b) Kind of water used: c) Average temperature of the water bath during the experiment: d) Average room temperature during the experiment (estimate if necessary): e) Duration of yeast solutions exposure to bath: 2. List your results in Tables 3.1 - 3.4. Table 3.1. Independent variables and experimental conditions. Bottl e Sugar Yeast Water Yeast solution height (in cm) To be heated in warm water bath? 1 1 teasp 2 teasp ¼ cup No. Leave this bottle at room temp. 2 1 teasp 2 teasp ¼ cup Yes. 3 1 teasp 2 teasp ¼ cup Yes. Replicates bottle #2 Updated May 2015
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4 1/3 teasp 2 teasp ¼ cup Yes. 5 No Sugar 2 teasp ¼ cup Yes. 6 ¼ cup Table 3.2. Observations of dependent variables. Bottle Balloon size Yeast growth Other observations 1 2 3 4 5 6 Table 3.3. Balloon size and solution height measurements. Bottl e Circumferenc e, C (cm) Uncertainty in C, ΔC Radius (long axis, R; cm) Uncertainty in R, ΔR New height of yeast solution (in cm) 1 2 3 4 5 6 3. In Table 3.4, record yeast growth and estimated volume of each balloon on Bottles 1- 6. a. Yeast growth = New height (in Table 3.3) - Original height (in Table 3.1) b. If the balloon did not inflate, it has a volume of zero. c. To estimate the volume of each balloon, use the following formula for the approximate volume of an ellipsoid with a horizontal circumference C and long axis radius R (from Table 3.3): Updated May 2015
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Unit 1 Home Lab 4: GeneTcs and DNA Points: 40p Name: Purpose (4p) Lab Summary (6p) Lab Answers (14p) 1. Describe what you can see in the final DNA extraction solution. Is the precipitant bubbly or stringy? Does it stick together or does it form many islands? 2. List your phenotype for the tongue rolling, ear attachment, and hitch-hiker thumb traits in Table 4.1. Use the following notation: a) If you can roll your tongue, then your phenotype is R. If you cannot, then your phenotype is r. b) If your earlobes are unattached, then your phenotype is U. If your earlobes are attached, then your phenotype is u. c) If you do not have a hitch-hiker thumb, then your phenotype is H. If you do have a hitch-hiker thumb, then your phenotype is h. Use the information above to determine your possible genotypes and record them in Table 4.1. Notice that the phenotype for a given trait is recorded with a single letter, whereas the genotype requires two letters per trait. Then, using what you have figured about your genotype, infer the different possible genotypes that your parents could have had. For instance, if you Updated May 2015
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determine that your possible genotype for earlobe attachment is UU or Uu, then the possible parental genotypes are: Possible parents of UU: UU ×UU; UU ×Uu; Uu × Uu Possible parents of Uu: UU ×Uu; UU × uu; Uu × Uu; Uu × uu For this question, do not ask your parents about their phenotypes! You will do this in question 3. Question 2 is an exercise in inference based on your understanding of genetics. Table 4.1. Personal phenotype and genotype; inferred possible parental genotypes. Trait Your Phenotyp e Your possible Genotypes Inferred possible parental genotypes Tongue rolling (R or r) Earlobe attachment (U or u) Hitch-hiker thumb (H or h) 3. Complete Table 4.2 for you, any blood relatives that you can ask (i.e., parents, siblings, children, etc.), and at least five unrelated “Others” (e.g., spouse, friends, co- workers, etc.). As before, phenotypes for a given trait are recorded with a single letter. You may wish to report separately on your children and spouse in Table 4.3. Table 4.2. Observed parental, sibling, and other’s phenotypes, Trait Mother’s Phenotyp e Father’s Phenotyp e Relatives’ Phenotype( s) Others’ Phenotype(s) Updated May 2015
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Lab Report 1
Purpose
The purpose of this lab is familiarize oneself and understand the different form of
measurements and conversions used in biology. To further the understanding of
measuring by...

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