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Bio Test Assignment Part 1: MCQs 1. Virus-mediated transfer of cellular gene±c material from one bacterial cell to another by means of virus par±cles is called: (A) Transduc±on Transduc±on is among the three basic mechanism of gene±c exchange in Bacteria. It allows the movement of gene±c informa±on from a donor cell to a recipient just like transforma±on and conjuga±on. Unlike the other mechanisms, however, transduc±on requires the par±cipa±on of a type of virus called a bacteriophage in order to accomplish this movement. 2. One strand of double-stranded DNA is mutated, changing all cytosines to uracils. AFer one round of replica±on of the mutated DNA strand, the mel±ng temperature of the resul±ng DNA will: (A) Be higher N/A 3. The Southern blo²ng technique is used for: (B) The detec±on of DNA fragments on membranes by a radioac±ve DNA probe Southern blo²ng technique is used to determine a speci³c DNA in a mixture through agarose gel electrophoresis. This hybridiza±on technique is used by researchers to determine the presence of certain nucleo±de sequences in a DNA sample. 4. Superoxide dismutase is an important enzyme for maintenance of red blood cells and is defec±ve insome neurodegenera±ve diseases. What does this enzyme do? (A) Catalyzes the conversion of O2- to H2O2 and O2 Superoxide dismutase is an enzyme that catalyzes the conversion of superoxide into hydrogen peroxide and oxygen. 5. Carboxymethyl cellulose is: (C) an anion-exchange matrix Carboxymethyl cellulose (CMC) also called cellulose gum is a cellulose deriva±ve with carboxymethyl groups (-CH2-COOH) bound to some of the hydroxyl groups of the glucopyranose monomers that make up the cellulose backbone. It is oFen used as its sodium salt, sodium carboxymethyl cellulose. 6. Targeted suppression of gene expression is achieved by: (C) RNAi RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression, typically by causing the destruc±on of speci³c mRNA molecules.
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7. Cystc fbrosis is due To: (B) DeFectve LDL recepTor Cystc fbrosis is a herediTary conditon, caused by a deFectve gene. A deFecT in The C±²R gene causes cystc fbrosis. ²hereFore deFectve LDL recepTor is The correcT answer. 8. ²he main di³erence beTween actve TransporT and FaciliTaTed di³usion is ThaT: (C) In actve TransporT, energy is consumed To move molecules againsT a concenTraton gradienT. Actve TransporT requires energy in The Form oF A²P in order To move molecules againsT a concenTraTed gradienT. 9. in compettve inhibiton (A) Km increases, V max consTanT Compettve inhibiTors bind To The actve siTes oF an enzyme and decrease The amounT oF binding oF The subsTraTe or ligand To enzyme. ²he resulT is ThaT The Km is increased and Vmax remains The same. UltmaTely, The chemical reacton can be reversed by increasing concenTraton oF subsTraTe. 10. ²he endogenous G²Pase actviTy oF G-proTeins serves To: (D) Hydrolyze G²P reTurning The G proTein To a pre-stmulaTed level oF actviTy. Cellular responses To signals are always reversible. Ras as a G proTein is actvaTed by exchanging G²P For bound GDP. ²he endogenous G²Pase oF Ras degrades The G²P back To GDP, and hence inactvaTes The proTein. ParT 2: Essay • Child
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Bio Test Assignment
Part 1: MCQs
1. (A) Transduction
Transduction is among the three basic mechanism of genetic exchange in Bacteria. It allows
in the genetic information movement from a donor cell...

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