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Virtual Lab The Immunology Virtual Lab Student Worksheet IMMUNOLOGY VIRTUAL LAB WORKSHEET INTRODUCTION Go to

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www.BioInteractive.org Published January 2015 Page 1 of 6 Student Worksheet Virtual Lab The Immunology Virtual Lab IMMUNOLOGY VIRTUAL LAB WORKSHEET INTRODUCTION Go to http://www.hhmi.org/biointeractive/immunology-virtual-lab. Start the Virtual Lab and maximize the screen if you wish. Answer the following questions in the spaces provided. DIAGNOSIS 1. Where are antibodies found? _____________________________________________________________________________________ _____________________________________________________________________________________ 2. How can they be used in the laboratory? _____________________________________________________________________________________ _____________________________________________________________________________________ 3. What does ELISA stand for? _____________________________________________________________________________________ _____________________________________________________________________________________ 4. What are ELISA assays used for in labs? _____________________________________________________________________________________ _____________________________________________________________________________________ 5. What are the three important limitations of an ELISA? Explain each. Limitation Explanation
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www.BioInteractive.org Published January 2015 Page 2 of 6 Student Worksheet Virtual Lab The Immunology Virtual Lab BACKGROUND 1. What test can be used to determine whether a patient has an infectious or autoimmune disease? _____________________________________________________________________________________ _____________________________________________________________________________________ 2. What does a positive result indicate? _____________________________________________________________________________________ _____________________________________________________________________________________ 3. The watery fluid of the blood is called ____________________. 4. What is allowed to react with the target antigen? _____________________________________________________________________________________ _____________________________________________________________________________________ 5. Detection is possible when _________________________________________________. 6. Once isolated, the secondary antibody can be ____________________________________ ___________________________________________________________________ 7. What is the signaling system? _____________________________________________________________________________________ _____________________________________________________________________________________ 8. What happens when the appropriate chemical (substrate) is added? _____________________________________________________________________________________ _____________________________________________________________________________________ 9. How is the test quantified? _____________________________________________________________________________________ _____________________________________________________________________________________ 10. What does the amount of color reflect? _____________________________________________________________________________________ _____________________________________________________________________________________
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