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Imidazole hydrochloride ( PK= = 6.95 ) is an acid salt of the conjugate base imidazole ! Imidazole plays an important role in protein biochemistry...

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can I have help on the two part question like filling out the table on the first part and an explanation for part b

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1 . Imidazole hydrochloride ( PK= = 6.95 ) is an acid salt of the conjugate base imidazole !
Imidazole plays an important role in protein biochemistry since it is a component of the
amino acid histidine . Solutions of imidazole are frequently used in protein purification ,
the process by which a single protein is isolated from an intracellular " soup " of biological
molecules
( 2) In your structural biology lab , you have access to I'M stock solutions of imidazole*
hydrochloride ( I'mIt ) and imidazole ( I'm ) . One afternoon , you constructed a series of
trials in which you mixed these two stock solutions with water to form new 100 m]
solutions with varied [I'm]It ] and [I'm ] . You also measured the pil of each solution .
In the process , you splashed water onto your lab notebook , obscuring some of your
dutifully recorded values .
Luckily , you have enough information to reconstruct your table . Fill in the missing
values below . Use the remainder of Page I and Page 2 to show your work .
Conc. of I'm HIT
Conc . of I'm in
Vol . of 1 M
Vol . of 1 M I'm
in mixed solution
mixed solution
I'mHIT stock
stock solution
Volume of H20
PH
( MM )
( MM )
solution ( MI )
( m [ )
added ( m [ )
200
200
200
800
80
100
200
10
20
70
200
20
6.65
CHEM 132
Chapter 17: Additional Aspects of Acid- Base Equilibria
Page 1

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( b ) Explain in your own words why the pil of the mixed solution containing imidazole and
imidazole hydrochloride does not change significantly when you vary the concentration
of either the weak acid or conjugate base . Justify your conclusion quantitatively and
qualitatively , considering both percent ionization and Le Chatelier's principle .
CHEM 132
Chapter 17: Additional Aspects of Acid-Base Equilibria
Page 3|

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