Spectrophotometry #2: Analysis of a Mixture (S. Crimson Hawk) Pre-laboratory Assignment. In Parts I and II, you will measure the absorbance of stock...
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Spectrophotometry #2: Analysis of a Mixture (S.S. Crimson Hawk)
Pre-laboratory Assignment. Based on your lab instructor's directions, read through the
experiment and a) answer the questions below; or b) complete the pre-lab assignment at Sapling
Learning online.
1. In Parts I and II, you will measure the absorbance of stock solutions of copper ion and nickel
ion. Copper (II) ions in aqueous solution are typically blue. Nickel (II) ions in aqueous solutions
are typically green.
a. What color of light is being absorbed in a Cut solution?
b. Around what wavelength do you expect a Cut solution to absorb?
c. What color of light is being absorbed in a Nit solution?
d. Around what wavelength do you expect a Nit solution to absorb?
2. In the previous lab, you measured the absorbance values of a dye mixture. Recall how the
spectrum of the mixture compared to the spectra of individual dyes. With that in mind, how do
you expect the spectrum of a mixture of copper and nickel ions compare to the spectra of
individual copper and nickel ions in solution?
formula:
3. A plot of (Acoin / ANi) versus (Acu / ANi) (remember, y vs. x) gives a regression line with the
y = 0.226x + 0.457
If the Cust standard concentration is 0.528 M and the Nit standard concentration is 0.976 M,
what are the concentrations of Cut and Nit in the coin solution? Show your work.

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