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1) What different components will do you want to add to your PCR? a) Template DNA and DNA Polymerase b) Template DNA and Primers c) Template DNA, Primers and DNA Polymerase 2)Which primers will you use? a) 5'-ATGGCT-3' only b) 5'-ATGGCT-3' and 5'-TTAGCC-3' c) 5'-ATGGCT-3' and 5'-AATCGG-3' 3) How many PCR cycles should you set the thermocycler for to obtain 128 TOTAL strands of DNA at the end of the reaction (this includes the template strands that you started with)? a) 5 b) 6 c) 7 4)To analyze the PCR you need to make a DNA gel. What do you want to add to make the gel? a) Agarose and water b)Agarose and TAE buffer c) Agarose, TAE buffer and Ethidium bromide

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a) What different components will do you want to add to your FCK? b) Which primers will you use? c) How many PCR cycles should you set the thermocycler for to obtain 128 TOTAL strands of DNA at the end of the reaction (this includes the template strands that you started with)? d) To analyze the PCR you need to make a DNA gel. What do you want to add to make the gel?

This question was created from Lab #2 PCR.pdf

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Subject: Chemistry, Science
a) What different components will do you want to add to your FCK? b) Which primers will you use? c) How many PCR cycles should you set the...
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