View the step-by-step solution to:

Question

This question was created from Lab #2 PCR.pdf https://www.coursehero.com/file/32655930/Lab-2-PCRpdf/

32655930-352222.jpeg

1) What different components will do you want to add to your PCR? a) Template DNA and DNA Polymerase b) Template DNA and Primers c) Template DNA, Primers and DNA Polymerase 2)Which primers will you use? a) 5'-ATGGCT-3' only b) 5'-ATGGCT-3' and 5'-TTAGCC-3' c) 5'-ATGGCT-3' and 5'-AATCGG-3' 3) How many PCR cycles should you set the thermocycler for to obtain 128 TOTAL strands of DNA at the end of the reaction (this includes the template strands that you started with)? a) 5 b) 6 c) 7 4)To analyze the PCR you need to make a DNA gel. What do you want to add to make the gel? a) Agarose and water b)Agarose and TAE buffer c) Agarose, TAE buffer and Ethidium bromide

32655930-352222.jpeg

a) What different components will do you want to add to your FCK?
b) Which primers will you use?
c) How many PCR cycles should you set the thermocycler for to obtain 128 TOTAL strands of
DNA at the end of the reaction (this includes the template strands that you started with)?
d) To analyze the PCR you need to make a DNA gel. What do you want to add to make the gel?

Top Answer

Sign up to view the full answer

Why Join Course Hero?

Course Hero has all the homework and study help you need to succeed! We’ve got course-specific notes, study guides, and practice tests along with expert tutors.

  • -

    Study Documents

    Find the best study resources around, tagged to your specific courses. Share your own to gain free Course Hero access.

    Browse Documents
  • -

    Question & Answers

    Get one-on-one homework help from our expert tutors—available online 24/7. Ask your own questions or browse existing Q&A threads. Satisfaction guaranteed!

    Ask a Question