Chromatography and Liquid Chromatography Experiment 18 9. You want to determine the amount of mobile phase needed to wash a particular compound off...
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What is the purpose of marking each component half off the column in liquid chromatography when calculating the

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Chromatography and Liquid Chromatography
Experiment 18
9.
You want to determine the amount of mobile phase needed to wash a particular
compound off the column. This determination begins by noting the level of mobile
phase when the first compound is halfway off the column (see following figure).
When you see this, make another mark on your column. This will be Point 2, This
distance between the initial level of the mobile phase (Point 1) and the level when
the first compound is halfway off the column (Point 2) is dl. Repeat this type of
measurement for each color (d2, d3, etc.) that comes off the column.
the following:
The chromatographyc progress of one component through the column is shown in
Step 5
Step 6
Step 8
Step 9
Hayden-McNeil, LLC
point 1
point 1
point 1
mobile
phase
sample
point 2
mobile phase
frit
level when
sample
compound Is
moving
halfway off the
column
octadecyl-silica
5-6 cm
. .
houpdar.
nonpolar
attracts
Wp, band
width of
Iin the
sample
column
sample band
half off the
column
D- H

qa_attachment_1570057874598.jpg

Q10.
You placed a mark when each component was half off the column in order to
ultimately calculate the retention volume. Why do you think you took your
measurement here as opposed to the start of the component leaving the column? Or
the end?

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