(5 marks) Write a detailed stepwise procedure ( with specific volumes and masses) for determining the polarity of lysozyme active site empirically....
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(5 marks) Write a detailed stepwise procedure ( with specific volumes and masses) for determining the polarity of
lysozyme active site empirically. For any spectra that you take, suggest an appropriate wavelength range (at least
50 nm of the expected wavelength maximum) for the measurement. You are required to use all the following
equipment and materials:
4 mg L-tryptophan (solid powder)
Fluorimeter
Lysozyme solution
Scanning spectrophotometer
150 mL of each solvent selected in Question 3-B
4 mL cuvettes
You also have access to the standard equipment basket from your locker.

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