View the step-by-step solution to:

The enzymes used in this experiment are only a partially purified pyruvate kinase preparation.

The enzymes used in this experiment are only a partially purified pyruvate kinase preparation. This is because complete purification would strip away co-factors and co-enzymes that are necessary for enzyme activities. However, all substrates used in the assay are pure.
i) Can you suggest any possible contaminating enzyme that might directly interfere with this assay? (Hint: Assume no membranes are present in the preparation.)
ii) Give a balanced equation for any reaction catalysed by a contaminating enzyme, including all cofactors and metal ion requirements.
iii) If present, how would this contaminating enzyme affect the assay?
iv) What assay would you carry out to determine the presence of such a contaminating enzyme this partially purified preparation?

This question was asked on Mar 21, 2010.

Recently Asked Questions

Why Join Course Hero?

Course Hero has all the homework and study help you need to succeed! We’ve got course-specific notes, study guides, and practice tests along with expert tutors and customizable flashcards—available anywhere, anytime.

-

Educational Resources
  • -

    Study Documents

    Find the best study resources around, tagged to your specific courses. Share your own to gain free Course Hero access or to earn money with our Marketplace.

    Browse Documents
  • -

    Question & Answers

    Get one-on-one homework help from our expert tutors—available online 24/7. Ask your own questions or browse existing Q&A threads. Satisfaction guaranteed!

    Ask a Question
  • -

    Flashcards

    Browse existing sets or create your own using our digital flashcard system. A simple yet effective studying tool to help you earn the grade that you want!

    Browse Flashcards